Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Genomic heterogeneity and host recovery of isolates of Malva vein clearing virus
Year:2009
Research Areas
  • Molecular biology
Information
Abstract
Malva vein clearing virus (MVCV), a tentative species of the genus Potyvirus, was identified as the causal agent of viral symptoms in Malva sp. weed plants. Amplified viral genomic fragments corresponding to approximately 20% of the 3 terminal region of its genome were obtained using non-species specific, genus-specific reagents. The sequences of the PCR fragments were determined. BLAST and phylogenetic analyses of the deduced amino acid sequence indicated that MVCV is a distinct species of the genus Potyvirus and close to Pea seed-borne mosaic virus (PSbMV) with which it forms a newphylogenetic cluster within the genus. The results show that MVCV is a definitive member of the Potyvirus genus. Specific MVCV PCR primers were designed and validated as diagnostic tools, and used to assess the variability of the species. Much variation was found and this was not correlated with either the geographical origin of the isolates, or the severity of the symptoms. Recovery from viral symptoms was observed in natural and experimental hosts. Tests in experimental hosts showed that it was a true viral recovery, in that the virus was absent and the recovered tissues could not be infected. This is the first reported example of true viral recovery of a potyvirus in a natural system.
International
Si
JCR
Si
Title
VIRUS RESEARCH
ISBN
0168-1702
Impact factor JCR
2,429
Impact info
Volume
Journal number
0
From page
91
To page
97
Month
ENERO
Ranking
Participants
  • Participante: Angeles Tourino
  • Participante: Pablo Lunello
  • Autor: Flora Sanchez Sanchez (UPM)
  • Participante: Yolanda Nunez
  • Autor: Fernando Ponz Ascaso (UPM)
Research Group, Departaments and Institutes related
  • Creador: Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)