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Memorias de investigación
Communications at congresses:
Phylogenetic analysis of the begomovirus population infecting wild pepper (Capsicum annuum var. aviculare) in México.
Year:2010
Research Areas
  • Plants and animal biology and ecology
Information
Abstract
The plant virus genus Begomovirus is characterized by geminate particles and, mostly, with a bipartite single-strand circular DNA genome (DNA A and DNA B). Begomoviruses are transmitted by whiteflies (Bemisia tabaci complex) and cause important diseases in tropical and temperate areas, where they are typical examples of emergent diseases. The possibility of an origin of crop-infecting begomoviruses in wild crop relatives, and their impact in wild plant populations, has been only analyzed in East Africa. Here, we address these issues in wild pepper (Capsicum annuum var. aviculare), the ancestor of chilli pepper, known as ¿chiltepín¿ or ¿chile piquin¿, which in Mexico is frequently infected by Pepper golden mosaic (PepGMV) and Pepper huasteco yellow vein (PHYVV) begomovirus. For this, the genetic structure of the chiltepín-infecting population of these viruses was analyzed. Chiltepín populations were sampled in habitats with different degrees of anthropogenic influence, from natural forests to crops in local home gardens. Samples were collected along the distribution area of chiltepin in Mexico between 2001 and 2007. PepGMV and PHYVV occurred frequently in mixed infection, which might facilitate the generation of new genotypes by recombination. Therefore we analyzed the occurrence of recombination, with no evidence of recombinants between PepGMV and PHYVV. However, there was evidence of recombinant genotypes of PepGMV or PHYVV, with potential sites of recombination occurring in the open reading frames encoding the replication protein, coat protein and the non-coding intergenic region. The genetic structure of PepGMV and PHYVV was based on the sequence of a fragment of the coat protein gene of 124 isolates. The results showed that the population of neither virus was genetically differentiated, neither by geographical location nor by habitat type. In a second approach, 72 full-length DNA-A sequences were determined and analyzed, which detected a spatial structure of the population of both viruses, with the subpopulation from Yucatán being differentiated from that of other regions. These results contrast with the population structure of the host plant, which is highly differentiated according to the origin of samples from various biogeographical regions, and suggests a recent epidemiological expansion of PepGMV and PHYVV on chiltepín populations, in parallel with its emergence in crops.
International
No
Congress
II Reunión de la Red Nacional de Virología de Plantas REVIPLANT 2010
960
Place
Puerto de Santa María (Cádiz, España)
Reviewers
Si
ISBN/ISSN
00-0000-000-0
Start Date
08/03/2010
End Date
10/03/2010
From page
71
To page
71
Libro de resúmenes REVIPLANT
Participants
  • Autor: Fernando Garcia-Arenal Rodriguez (UPM)
  • Participante: MÓNICA BETANCOURT
  • Autor: Manuel Alfredo Rodelo Urrego (UPM)
  • Autor: Aurora Fraile Perez (UPM)
  • Participante: DANIEL PIÑERO (Universidad Nacional Autónoma de Mexico)
  • Autor: Jesus Israel Pagan Muñoz (UPM)
  • Autor: Maria Angeles Ayllon Talavera (UPM)
  • Autor: Pablo Gonzalez Jara (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Patología Vegetal
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
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