Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Influence of hormonal and non-hormonal estrus synchronization methods on follicular and oocyte quality in primiparous lactating does at early postpartum period.
Year:2010
Research Areas
  • Livestock
Information
Abstract
High-yield lactating does need effective estrus synchronization methods to improve their reproductive outcome by enhancing ovarian function. The aim of the current work was to analyze ovarian follicular and oocyte characteristics of hormonal and nonhormonal estrus synchronization regimes in primiparous lactating rabbit does (Oryctolagus cuniculus) in the early postpartum period (Day 11). Females were randomly treated with either (1) a hormonal standard treatment with 25 IU equine chorionic gonadotropin (eCG) 48 h before artificial insemination (eCG group) or (2) an alternative nonhormonal treatment consisting of doelitter separation 24 h before artificial insemination (Bio group). No significant differences were found in serum estradiol and progesterone concentrations between experimental groups. During the histologic study, the Bio group presented a higher number of primordial (P < 0.05) and primary follicles (P = 0.07) compared with that of the eCG group, whereas secondary and antral follicular populations were similar. Rates of late atretic follicles assessed by the terminal deoxynucleotidyl transferase¿mediated dUTP nickend labeling technique were not different between treatments, but the eCG group showed a significantly higher number of midatretic follicles compared with that of the Bio group. Nuclear in vitro oocyte maturation (IVM), measured as metaphase II rate, and in vitro steroidogenic response of cumulus-oocyte complexes, measured by ELISA, did not show significant differences between treatments. However, confocal study showed that cytoplasmic maturation of oocytes, in terms of cortical granule migration rate, was significantly higher in the Bio group compared with that after the eCG treatment. In conclusion, transient doe-litter separation seems to improve ovarian response in terms of follicular health and oocyte competence compared with that after the eCG treatment. Therefore, a 24-h-long transient weaning could be an alternative nonhormonal method for synchronizing estrus in primiparous lactating rabbit does inseminated in the early postpartum period.
International
Si
JCR
Si
Title
THERIOGENOLOGY
ISBN
0093-691X
Impact factor JCR
2,073
Impact info
Volume
73
Journal number
From page
26
To page
35
Month
ENERO
Ranking
Participants
  • Autor: Maria del Pilar Garcia Rebollar (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Producción Animal
S2i 2019 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
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