Observatorio de I+D+i UPM

Memorias de investigación
Artículos en revistas:
Influence of leptin on in vitro maturation and steroidogenic secretion of cumulus-oocyte complexes through Jak2/STAT3 and MEK ? pathways in the rabbit model.
Año:2010
Áreas de investigación
  • Ganaderia
Datos
Descripción
Extreme body mass indexes may impair reproductive outcome in assisted reproductive technologies. Leptin reflects the amount of body fat and could act as a modulator of oocyte quality through activation of specific transcription factors. The aim of this work was to establish whether: 1) leptin influences meiotic and cytoplasmic oocyte maturation; 2) STAT3 and MAPK mediate the effects of leptin and 3) leptin modulates steroid secretion by cumulus¿oocyte complexes (COC) during in vitro maturation (IVM). We confirmed immunolocalisation of leptin receptor in oocytes, cumulus/granulosa cells during the peri-ovulatory period. The confocal study showed that COC supplemented with 1, 10 and 100 ng/ml leptin had a significantly higher metaphase II (MII) percentage than those IVM without leptin (P!0.05) and a similar MII index compared to the group supplemented with 10% FCS. Leptin did not increase the percentage of cytoplasmically matured oocytes in terms of cortical granule migration rate, whereas a significantly higher index was found in the FCS group (P!0.001). Oestradiol concentrations in spent media were higher in the FCS group compared to other treatments (P!0.001). Leptin-stimulated nuclear oocyte maturation was significantly impaired when leptin-induced JAK2/STAT3 and MEK 1/2 activation was suppressed by the inhibitors (P!0.001). Steroid secretion of COC was not affected by leptin activation of JAK2/STAT3 or MEK 1/2 pathways. In conclusion, JAK2/STAT3 and MEK 1/2 pathways mediate the enhancement of nuclear oocyte maturation by leptin; however, neither cytoplasmic oocyte maturation nor steroidogenic response of COC were improved in the present rabbit model.
Internacional
Si
JCR del ISI
Si
Título de la revista
REPRODUCTION
ISSN
1470-1626
Factor de impacto JCR
2,579
Información de impacto
Volumen
139
DOI
Número de revista
Desde la página
523
Hasta la página
532
Mes
ENERO
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Nuria Nicodemus Martin (UPM)
  • Autor: Maria del Pilar Garcia Rebollar (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Producción Animal
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