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Descripción
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| We present a filtering technique for extracting nuclei trajectories in 4D microscopy images of zebrafish embryogenesis based on a spatio-temporal granulometric analysis. Previous work showed that the morphological reconstruction operator with 4D structuring elements (SEs) allows us to directly recover nuclei trajectories as tubular-like structures in 4D. The performance and usability of this method is determined by two main factors. The first factor is the "intra-trajectory distance" -Dintra-, this means the maximum displacement of a nucleus between time frames, which is a function of the time step between image acquisitions, the spatial resolution and the cell speed. The other factor is the "inter-trajectory distance" -Dinter-, standing for the minimum distance between a nucleus and its neighbors in the spatio-temporal domain and is function of the microscopy resolution and the observed biological phenomena. | |
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Internacional
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Si |
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Entidad
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Université René Descartes |
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Lugar
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Paris |
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Páginas
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49 |
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Referencia/URL
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2nd conference of the annual series ¿Morphogenesis in Living Systems¿ |
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Tipo de publicación
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Libro de resúmenes |