Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Identification of water stress genes in Pinus pinaster Ait. by controlled progressive stress and suppression-subtractive hybridization
Year:2012
Research Areas
  • Natural sciences and health sciences,
  • Molecular biology,
  • Plant genetics
Information
Abstract
Climate change is a major challenge particularly for forest tree species, which will have to face the severe alterations of environmental conditions with their current genetic pool. Thus, an understanding of their adaptive responses is of the utmost interest. In this work we have selected Pinus pinaster as a model species. This pine is one of the most important conifers (for which molecular tools and knowledge are far more scarce than for angiosperms) in the Mediterranean Basin, which is characterised in all foreseen scenarios as one of the regions most dras- tically affected by climate change, mainly because of increasing temperature and, particularly, by increasing drought. We have induced a controlled, increasing water stress by adding PEG to a hydroponic culture.We have generated a subtractive library, with the aim of identifying the genes induced by this stress and have searched for the most reliable expressional candidate genes, based on their overexpression during water stress, as revealed by microarray analysis and con!rmed by RT-PCR. We have selected a set of 67 candidate genes belonging to different functional groups that will be useful molecular tools for further studies on drought stress responses, adaptation, and population genomics in conifers, as well as in breeding programs.
International
Si
JCR
Si
Title
Plant Physiology and Biochemistry
ISBN
0981-9428
Impact factor JCR
2,402
Impact info
Volume
doi:10.1016/j.plaphy.2011.09.022
Journal number
50
From page
44
To page
53
Month
ENERO
Ranking
Participants
  • Autor: Pedro Perdiguero Jimenez (UPM)
  • Autor: Maria Carmen Collada Collada (UPM)
  • Autor: Mª Carmen Barbero Ameller (UPM)
  • Autor: Gloria García Casado (Centro Nacional de Biotecnología CNB)
  • Autor: María Teresa Cervera Goy (UPM)
  • Autor: Alvaro Soto De Viana (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Genética y Fisiología Forestal
  • Departamento: Biotecnología
S2i 2019 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)