Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Selectivity of diacylhydrazine insecticides to the predatory bug Orius laevigatus: In vivo and modeling/docking experiments
Year:2012
Research Areas
  • Molecular, cellular and genetic biology,
  • Entomology,
  • Agriculture,
  • Means of agricultural production,
  • Insecticide
Information
Abstract
BACKGROUND: Knowledge of pesticide selectivity to natural enemies is necessary for a successful implementation of biological and chemical control methods in integrated pest management (IPM) programs. Diacylhydrazine (DAH)-based ecdysone agonists also known as molting-accelerating compounds (MACs) are considered a selective group of insecticides, and their compatibility with predatory Heteroptera, which are used as biological control agents, is known. However, their molecular mode of action has not been explored in beneficial insects such as Orius laevigatus (Fieber) (Hemiptera: Anthocoridae). RESULTS: In this project in vivo toxicity assays demonstrated that the DAH-based RH-5849, tebufenozide and methoxyfenozide have no toxic effect against O. laevigatus. The ligand-binding domain (LBD) of the ecdysone receptor (EcR) of O. laevigatus was sequenced and a homology protein model was constructed which confirmed a cavity structure with 12 ?-helixes, harboring the natural insect molting hormone 20-hydroxyecdysone. However, docking studies showed that a steric clash occurred for the DAH-based insecticides due to a restricted extent of the ligand-binding cavity of the EcR of O. laevigatus. CONCLUSIONS: The insect toxicity assays demonstrated that MACs are selective for O. laevigatus. The modeling/docking experiments are indications that these pesticides do not bind with the LBD-EcR of O. laevigatus and support that they show no biological effects in the predatory bug. These data help in explaining the compatible use of MACs together with predatory bugs in IPM programs. Keywords: Orius laevigatus, selectivity, diacylhydrazine insecticides, ecdysone receptor, homology modelling, docking studies.
International
Si
JCR
Si
Title
Pest Management Science
ISBN
1526-4998
Impact factor JCR
2,251
Impact info
Volume
18
Journal number
2
From page
1586
To page
1594
Month
JULIO
Ranking
Participants
  • Autor: Fermín Amor Parrilla (UPM)
  • Autor: Olivier Christiaens (Ghent University)
  • Autor: Paloma Bengochea Budia (UPM)
  • Autor: Maria Pilar Medina Velez (UPM)
  • Autor: Pierre Rougé (Université Paul Sabatier)
  • Autor: Elisa Viñuela Sandoval (UPM)
  • Autor: Guy Smagghe (Ghent University)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Manejo Integrado de Plagas
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