Observatorio de I+D+i UPM

Memorias de investigación
Ponencias en congresos:
Utilización de bolsas ANKOM® en la determinación de la digestibilidad de la materia seca in vitro en conejos.
Año:2011
Áreas de investigación
  • Ganaderia
Datos
Descripción
The aim of this work was to compare the use of ANKOM® bags vs. crucibles to determine in vitro dry matter digestibility (ivDMD, performed in two or three steps corresponding to ileal and faecal digestibility, respectively) of four rabbit diets and six raw materials with a wide range of DM digestibility (from beet pectins to lignocellulose). Crucibles were compared to bags placed collectively (up to 30 in a 3.6 L Daisy jar in a rotating in vitro incubator system Daisy-II). Once digested, samples placed collectively were washed with water (40ºC, 30 min, rotating in Daisy system, and additionally rinsed two times 5 min. in water), etanol and 96% acetone (each one for 5 min.). Bag samples were corrected with blanks. Each analysis was done at three different times being replicated twice each time and using the average of each time and feed for statistical analysis. Values obtained for ivDMD in two steps were similar using crucibles or bags for the whole feeds (P = 0.44, 43.1 vs. 42.8%, respectively), with no interaction feed x method. However, ivDMD in three steps decreased in collective bags compared to crucibles (- 0.6 percentage units. P = 0.009). This narrow difference is probably detected due to the reduced variability observed in this determination. Besides, an interaction feed x method was found for three steps ivDMD (P = 0.002), as digestion in collective bags was lower than that of crucibles for sugar beet pulp, sunflower hulls and straw, with no effect on the other feeds and in none diet. In general, these differences were small, ranging between 2.0 to 2.6 percentage units. Correlation between two and three step ivDMD with crucibles and bags was very high (r ? 0.98. P < 0.001). These results suggest that ANKOM® bags can be used as an alternative to crucibles in ivDMD.
Internacional
No
Nombre congreso
XV Jornadas sobre Producción Animal, AIDA
Tipo de participación
960
Lugar del congreso
Zaragoza
Revisores
No
ISBN o ISSN
9788461500628
DOI
Fecha inicio congreso
17/05/2011
Fecha fin congreso
18/05/2011
Desde la página
201
Hasta la página
203
Título de las actas
ITEA, tomo I, XV Jornadas sobre Producción Animal, AIDA
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Javier Garcia Alonso (UPM)
  • Autor: Rosa Maria Carabaño Luengo (UPM)
  • Autor: David Menoyo Luque (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Producción Animal
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