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Influence of regeneration medium composition in survival and genetic stability of mint (Mentha x piperita) cryopreserved apices

Research Areas
  • Crop production

Cryopreservation techniques are an important tool for long term germplasm conservation, and are considered the most genetic stable long-term conservation method. To obtain a reliable conservation method it is important to establish an adequate regeneration procedure in order to guarantee the maximum survival and re-growth. The regeneration culture medium employed could be an important factor to consider. Although genetic stability after cryopreservation is supposedly guaranteed, genetic variation in cryopreserved material has been occasionally reported (Harding, 2004; Martín and González-Benito, 2005; Martín et al., 2011). The origin of this variation is usually attributed to the application of in vitro tissue culture procedures which are necessary before or/and during the cryopreservation process, instead of the cryopreservation treatment itself. The exposure to extreme physical conditions and to certain chemicals employed as cryoprotectants (e.g. DMSO) may result in physiological stress and, consequently, in genetic instability. One of the hypothesis to explain the effect of these stresses on the DNA variation found is that the injury produced by the cryopreservation process (action of cryoprotectants and/or oxidative stress) may cause the formation of free radicals, which could potentially lead to genetic alterations (Benson and Bremner, 2004). These stresses are somehow added to those imposed by the employment of in vitro techniques. Therefore, cryopreservation success has to deal with a balance between high survival rate and genetic stability. In order to evaluate these important factors, a study of survival and genetic stability (using molecular markers) after cryopreservation has been carried out in mint apices cryopreserved with the vitrification-droplet method, and regenerated on three different media.
IVCHB 2011 Biotechnological advances in in vitro horticultural breeding
Gante (Bélgica)
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Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Biodiversidad y conservación de recursos fitogenéticos
  • Departamento: Biología Vegetal