Observatorio de I+D+i UPM

Memorias de investigación
Artículos en revistas:
Wavelet-based image fusion in multi-view three-dimensional microscopy
Año:2012
Áreas de investigación
  • Tratamiento de imágenes médicas
Datos
Descripción
Motivation: Multi-view microscopy techniques such as Light-Sheet Fluorescence Microscopy (LSFM) are powerful tools for 3D + time studies of live embryos in developmental biology. The sample is imaged from several points of view, acquiring a set of 3D views that are then combined or fused in order to overcome their individual limitations. Views fusion is still an open problem despite recent contributions in the ?eld. Results: We developed a wavelet-based multi-view fusion method that, due to wavelet decomposition properties, is able to combine the complementary directional information from all available views into a single volume. Our method is demonstrated on LSFM acquisitions from live sea urchin and zebra?sh embryos. The fusion results show improved overall contrast and details when compared with any of the acquired volumes. The proposed method does not need knowledge of the system?s point spread function (PSF) and performs better than other existing PSF independent fusion methods. Availability and Implementation: The described method was implemented in Matlab (The Mathworks, Inc., USA) and a graphic user interface was developed in Java. The software, together with two sample datasets, is available at http://www.die.upm.es/im/software/SPIMFusionGUI.zip A public release, free of charge for non-commercial use, is planned after the publication of this article.
Internacional
Si
JCR del ISI
Si
Título de la revista
Bioinformatics
ISSN
1367-4803
Factor de impacto JCR
5,468
Información de impacto
Volumen
28
DOI
10.1093/bioinformatics/BTR609
Número de revista
Desde la página
238
Hasta la página
245
Mes
ENERO
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: José Luis Rubio Guivernau (UPM)
  • Autor: Vasily Gurchenkov (Institut des Systèmes complexes )
  • Autor: Miguel Angel Luengo Oroz (UPM)
  • Autor: Louise Duloquin (Institut des Systèmes complexes )
  • Autor: Paul Bourgine (Institut des Systèmes complexes )
  • Autor: Andres de Santos Lleo (UPM)
  • Autor: Nadine Peyrieras (Institut des Systèmes complexes )
  • Autor: Maria Jesus Ledesma Carbayo (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Departamento: Ingeniería Electrónica
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