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Descripción
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| Investigating cell dynamics during early zebra?sh embryogenesis requires speci?c image acquisition and analysis strategies. Multiharmonic microscopy, i.e., second- and third-harmonic generations, allows imaging cell divisions and cell membranes in unstained zebra?sh embryos from 1- to 1000-cell stage. This paper presents the design and implementation of a dedicated image processing pipeline (tracking and segmentation) for the reconstruction of cell dynamics during these developmental stages. This methodology allows the reconstruction of the cell lineage tree including division timings, spatial coordinates, and cell shape until the 1000-cell stage with minute temporal accuracy and micrometer spatial resolution. Data analysis of the digital embryos provides an extensive quantitative description of early zebra?sh embryogenesis. | |
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Internacional
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Si |
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JCR del ISI
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Si |
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Título de la revista
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Ieee Transactions on Image Processing |
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ISSN
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1057-7149 |
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Factor de impacto JCR
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2,606 |
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Información de impacto
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Datos JCR del año 2010 |
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Volumen
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DOI
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10.1109/TIP.2011.2177911 |
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Número de revista
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Desde la página
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2335 |
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Hasta la página
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2340 |
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Mes
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SIN MES |
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Ranking
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