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Descripción
Investigating cell dynamics during early zebra?sh embryogenesis requires speci?c image acquisition and analysis strategies. Multiharmonic microscopy, i.e., second- and third-harmonic generations, allows imaging cell divisions and cell membranes in unstained zebra?sh embryos from 1- to 1000-cell stage. This paper presents the design and implementation of a dedicated image processing pipeline (tracking and segmentation) for the reconstruction of cell dynamics during these developmental stages. This methodology allows the reconstruction of the cell lineage tree including division timings, spatial coordinates, and cell shape until the 1000-cell stage with minute temporal accuracy and micrometer spatial resolution. Data analysis of the digital embryos provides an extensive quantitative description of early zebra?sh embryogenesis.
Internacional	Si
JCR del ISI	Si
Título de la revista	Ieee Transactions on Image Processing
ISSN	1057-7149
Factor de impacto JCR	2,606
Información de impacto	Datos JCR del año 2010
Volumen
DOI	10.1109/TIP.2011.2177911
Número de revista
Desde la página	2335
Hasta la página	2340
Mes	SIN MES
Ranking

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• Creador: Departamento: Ingeniería Electrónica