Observatorio de I+D+i UPM

Memorias de investigación
Artículos en revistas:
Differential in vitro and in vivo effect of barley cysteine and serine protease inhibitors on phytopathogenic microorganisms
Año:2011
Áreas de investigación
  • Ciencias naturales y ciencias de la salud,
  • Biología molecular
Datos
Descripción
Protease inhibitors from plants have been involved in defence mechanisms against pests and pathogens. Phytocystatins and trypsin/a-amylase inhibitors are two of the best characterized protease inhibitor families in plants. In barley, thirteen cystatins (HvCPI-1 to 13) and the BTI-CMe trypsin inhibitor have been previously studied. Their capacity to inhibit pest digestive proteases, and the negative in vivo effect caused by plants expressing these inhibitors on pests support the defence function of these proteins. Barley cystatins are also able to inhibit in vitro fungal growth. However, the antifungal effect of these inhibitors in vivo had not been previously tested. Moreover, their in vitro and in vivo effect on plant pathogenous bacteria is still unknown. In order to obtain new insights on this feature, in vitro assays were made against different bacterial and fungal pathogens of plants using the trypsin inhibitor BTI-CMe and the thirteen barley cystatins. Most barley cystatins and the BTI-CMe inhibitor were able to inhibit mycelial growth but no bacterial growth. Transgenic Arabidopsis plants independently expressing the BTI-CMe inhibitor and the cystatin HvCPI-6 were tested against the same bacterial and fungal pathogens. Neither the HvCPI-6 expressing transgenic plants nor the BTI-CMe ones were more resistant to plant pathogen fungi and bacteria than control Arabidopsis plants. The differences observed between the in vitro and in planta assays against phytopathogenic fungi are discussed.
Internacional
Si
JCR del ISI
Si
Título de la revista
Plant Physiology and Biochemistry
ISSN
0981-9428
Factor de impacto JCR
Información de impacto
Volumen
49
DOI
0.1016/j.plaphy.2011.03.012
Número de revista
Desde la página
1191
Hasta la página
1200
Mes
SIN MES
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Laura Carrillo
  • Autor: Ignacio Herrero
  • Autor: Ines Cambra Marin (UPM)
  • Autor: Maria Rosa Sanchez-Monge Y Laguna de Rins (UPM)
  • Autor: M. Isabel Diaz Rodriguez (UPM)
  • Autor: Manuel Martinez Muñoz (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Departamento: Biotecnología
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