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Memorias de investigación
Artículos en revistas:
Expression of a barley cystatin gene in maize enhances resistance against phytophagous mites by altering their cysteine-proteases
Año:2011
Áreas de investigación
  • Ciencias naturales y ciencias de la salud,
  • Biología molecular
Datos
Descripción
Phytocystatins are inhibitors of cysteine-proteases from plants putatively involved in plant defence based on their capability of inhibit heterologous enzymes. We have previously characterised the whole cystatin gene family members from barley (HvCPI-1 to HvCPI-13). The aim of this study was to assess the effects of barley cystatins on two phytophagous spider mites, Tetranychus urticae and Brevipalpus chilensis. The determination of proteolytic activity profile in both mite species showed the presence of the cysteine-proteases, putative targets of cystatins, among other enzymatic activities. All barley cystatins, except HvCPI-1 and HvCPI-7, inhibited in vitro mite cathepsin L- and/or cathepsin B-like activities, HvCPI-6 being the strongest inhibitor for both mite species. Transgenic maize plants expressing HvCPI-6 protein were generated and the functional integrity of the cystatin transgene was confirmed by in vitro inhibitory effect observed against T. urticae and B. chilensis protein extracts. Feeding experiments impaired on transgenic lines performed with T. urticae impaired mite development and reproductive performance. Besides, a significant reduction of cathepsin L-like and/or cathepsin B-like activities was observed when the spider mite fed on maize plants expressing HvCPI-6 cystatin. These findings reveal the potential of barley cystatins as acaricide proteins to protect plants against two important mite pests.
Internacional
Si
JCR del ISI
Si
Título de la revista
Plant Cell Reports
ISSN
0721-7714
Factor de impacto JCR
2,279
Información de impacto
Datos JCR del año 2010
Volumen
30
DOI
10.1007/s00299-010-0948-z
Número de revista
Desde la página
101
Hasta la página
112
Mes
SIN MES
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Laura Carrillo
  • Autor: Manuel Martinez Muñoz (UPM)
  • Autor: Koreen Ramessar
  • Autor: Ines Cambra Marin (UPM)
  • Autor: Pedro Castañera
  • Autor: Felix Ortego
  • Autor: M. Isabel Diaz Rodriguez (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Departamento: Biotecnología
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