Memorias de investigación
Research Publications in journals:
Isozyme systems as markers of genetic deterioration in stored seeds of Brassicaceae species
Year:2012
Research Areas
  • Natural sciences and health sciences
Information
Abstract
The main objective of this study was to determine if isozyme systems can be used as markers of genetic deterioration in Brassicaceae seed accessions under different storage conditions. Seed samples of Brassica oleracea, Cardaria draba, Erysimum cheiri, Iberis sempervirens and Rapistrum rugosum were stored for periods of 9 to 30 years at -10°C and 3-4% seed moisture content (long-term or LT conditions) and at 5°C and uncontrolled relative humidity (RH) (short-term or ST conditions). Starch Gel Electrophoresis (SGE) was used to analyse six enzyme systems oriented to determine the genetic deterioration of the accessions studied. The results obtained show that long-term storage conditions (LT) were extremely effective in maintaining the viability of seeds of the five Brassicaceae species studied. The final germination percentages reached by seeds from LT samples ranged from 75 to 100%, while the germination percentages of ST samples (except for B. oleracea) were very low (from 0 to 10%). Similar conclusions were obtained studying the integrity of electrophoretic bands for several isozymes. Two enzyme systems were of special interest: malate dehydrogenase and alcohol dehydrogenase.
International
Si
JCR
Si
Title
Seed Science And Technology
ISBN
0251-0952
Impact factor JCR
0,62
Impact info
Datos JCR del año 2011
Volume
Journal number
40
From page
365
To page
373
Month
SIN MES
Ranking
Participants
  • Autor: M. Itziar Josefina Aguinagalde Madariaga UPM
  • Autor: Silvia Maselli Departamento de Biología vegetal, Universidad Politécnica de Madrid
  • Autor: Felix Perez Garcia UPM
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Biodiversidad y conservación de recursos fitogenéticos
  • Departamento: Biología Vegetal