Observatorio de I+D+i UPM

Descripción
The main objective of this study was to determine if isozyme systems can be used as markers of genetic deterioration in Brassicaceae seed accessions under different storage conditions. Seed samples of Brassica oleracea, Cardaria draba, Erysimum cheiri, Iberis sempervirens and Rapistrum rugosum were stored for periods of 9 to 30 years at -10°C and 3-4% seed moisture content (long-term or LT conditions) and at 5°C and uncontrolled relative humidity (RH) (short-term or ST conditions). Starch Gel Electrophoresis (SGE) was used to analyse six enzyme systems oriented to determine the genetic deterioration of the accessions studied. The results obtained show that long-term storage conditions (LT) were extremely effective in maintaining the viability of seeds of the five Brassicaceae species studied. The final germination percentages reached by seeds from LT samples ranged from 75 to 100%, while the germination percentages of ST samples (except for B. oleracea) were very low (from 0 to 10%). Similar conclusions were obtained studying the integrity of electrophoretic bands for several isozymes. Two enzyme systems were of special interest: malate dehydrogenase and alcohol dehydrogenase.
Internacional	Si
JCR del ISI	Si
Título de la revista	Seed Science And Technology
ISSN	0251-0952
Factor de impacto JCR	0,62
Información de impacto	Datos JCR del año 2011
Volumen
DOI
Número de revista	40
Desde la página	365
Hasta la página	373
Mes	SIN MES
Ranking

Esta actividad pertenece a memorias de investigación

• Autor: M. Itziar Josefina Aguinagalde Madariaga (UPM)
• Autor: Silvia Maselli (Departamento de Biología vegetal, Universidad Politécnica de Madrid)
• Autor: Felix Perez Garcia (UPM)

• Creador: Grupo de Investigación: Biodiversidad y conservación de recursos fitogenéticos
• Departamento: Biología Vegetal