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Descripción
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| Cryopreservation protocols impose oxidative stresses to tissues that may affect plant regeneration and also to the genetic stability of cultures. The use of antioxidants at different steps of a cryopreservation protocol may reduce those negative effects. We have studied the use of antioxidants in an encapsulation-dehydration protocol with shoot apices of two different mint (Mentha xpiperita) genotypes (MEN 186 and MEN 198). The antioxidants were added in a preculture step, in which 0.3 M sucrose was also employed: 0.28 or 0.43 mM ascorbic acid, 0.16 or 0.24 mM glutathione (both supplemented to the preculture medium), or ?-tocopherol (spread on filter paper and placed on the medium). This treatment was applied to the apices previous to the encapsulation step of the cryopreservation protocol. After a 8-week recovery period, the regeneration response (organized growth) of apices not treated with antioxidants differed between genotypes: 37% in MEN 198 vs 13% in MEN 186. The use of ?-tocopherol increased the regeneration percentage in MEN 198 to 52%, although that increase was not so significant in MEN 186 (20%). The response of these two genotypes to the other antioxidant treatments was variable. The genetic stability of the recovered cultures was studied by RAPDs markers. Genetic differences among samples were detected in both genotypes. Near 50% of the studied samples showed the same genotype in both cases. A clear relationship between the quantity of variation accumulated and the type of sample was observed. | |
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Internacional
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Si |
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Nombre congreso
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Advances in Low Temperature Biology SLTB Conference 2012 |
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Tipo de participación
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960 |
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Lugar del congreso
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Londres (Reino Unido) |
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Revisores
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Si |
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ISBN o ISSN
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0143-2044 |
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DOI
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Fecha inicio congreso
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11/10/2012 |
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Fecha fin congreso
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12/10/2012 |
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Desde la página
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212 |
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Hasta la página
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212 |
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Título de las actas
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CryoLetters 34 |