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Descripción
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| One of the most reliable methods for long-term conservation of vegetatively propagated plants is cryopreservation, mainly due to its capability to guarantee the genetic stability of the preserved material during the process. However, the stresses suffered due to the treatments applied together with in vitro culture required for the regeneration of the plant material may result in some degree of genetic instability (1, 2 , 3). Mint (Mentha ?piperita) is an unfertile hybrid with clonal propagation. Two cryopreservation techniques (droplet-vitrification vs. encapsulation dehydration) were compared with the mint genotype MEN 186. The genetic analysis, using RAPD markers, revealed a higher variation in the regenerated plants from the encapsulation-dehydration protocol (4). In this work the genetic and epigenetic stability analyses of mint apices from a different genotype, MEN 198, cryopreserved under the same techniques and regenerated using three different media composition were carried out. Comparisons of the different treatments on the two mint genotypes are evaluated in order to obtain a protocol of cryopreservation technique and regeneration medium that could optimized the quality of the regenerated plants. | |
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Internacional
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Si |
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Nombre congreso
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Advances in Low Temperature Biology SLTB Conference 2012 |
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Tipo de participación
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960 |
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Lugar del congreso
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London (Reino Unido) |
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Revisores
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Si |
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ISBN o ISSN
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0143-2044 |
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DOI
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Fecha inicio congreso
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11/10/2012 |
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Fecha fin congreso
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12/10/2012 |
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Desde la página
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208 |
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Hasta la página
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208 |
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Título de las actas
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CryoLetters 34 |