Observatorio de I+D+i UPM

Memorias de investigación
Ponencias en congresos:
The effect of the cryopreservation technique, regeneration medium and genotype on the genetic stability of mint (Mentha xpiperita) cryopreserved apices
Año:2012
Áreas de investigación
  • Biodiversidad,
  • Producción vegetal
Datos
Descripción
One of the most reliable methods for long-term conservation of vegetatively propagated plants is cryopreservation, mainly due to its capability to guarantee the genetic stability of the preserved material during the process. However, the stresses suffered due to the treatments applied together with in vitro culture required for the regeneration of the plant material may result in some degree of genetic instability (1, 2 , 3). Mint (Mentha ?piperita) is an unfertile hybrid with clonal propagation. Two cryopreservation techniques (droplet-vitrification vs. encapsulation dehydration) were compared with the mint genotype MEN 186. The genetic analysis, using RAPD markers, revealed a higher variation in the regenerated plants from the encapsulation-dehydration protocol (4). In this work the genetic and epigenetic stability analyses of mint apices from a different genotype, MEN 198, cryopreserved under the same techniques and regenerated using three different media composition were carried out. Comparisons of the different treatments on the two mint genotypes are evaluated in order to obtain a protocol of cryopreservation technique and regeneration medium that could optimized the quality of the regenerated plants.
Internacional
Si
Nombre congreso
Advances in Low Temperature Biology SLTB Conference 2012
Tipo de participación
960
Lugar del congreso
London (Reino Unido)
Revisores
Si
ISBN o ISSN
0143-2044
DOI
Fecha inicio congreso
11/10/2012
Fecha fin congreso
12/10/2012
Desde la página
208
Hasta la página
208
Título de las actas
CryoLetters 34
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: M. Carolina Kremer Morales (UPM)
  • Autor: Maria Carmen Martin Fernandez (UPM)
  • Autor: I. González (UPM)
  • Autor: M. Elena Gonzalez Benito (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Biodiversidad y conservación de recursos fitogenéticos
  • Departamento: Biología Vegetal
S2i 2021 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)