|
Descripción
|
|
|---|---|
| Cryopreservation protocols impose oxidative stresses to tissues that may affect plant regeneration and also to the genetic stability of cultures. The use of antioxidants at different steps of a cryopreservation protocol may reduce those negative effects. We have studied the use of antioxidants in an encapsulation-dehydration protocol with shoot apices of two different mint (Mentha xpiperita) genotypes (MEN 186 and MEN 198). The antioxidants were added in a preculture step, in which 0.3 M sucrose was also employed: 0.28 or 0.43 mM ascorbic acid, 0.16 or 0.24 mM glutathione (both supplemented to the preculture medium), or ?-tocopherol (spread on filter paper and placed on the medium). This treatment was applied to the apices previous to the encapsulation step of the cryopreservation protocol. After a 8-week recovery period, the regeneration response (organized growth) of apices not treated with antioxidants differed between genotypes: 37% in MEN 198 vs 13% in MEN 186. The use of ?-tocopherol increased the regeneration percentage in MEN 198 to 52%, although that increase was not so significant in MEN 186 (20%). The response of these two genotypes to the other antioxidant treatments was variable. The genetic stability of the recovered cultures was studied by RAPDs markers. | |
|
Internacional
|
Si |
|
Nombre congreso
|
2nd Symposium on Horticulture in Europe (SHE 2012) |
|
Tipo de participación
|
960 |
|
Lugar del congreso
|
Angers, Francia |
|
Revisores
|
Si |
|
ISBN o ISSN
|
|
|
DOI
|
|
|
Fecha inicio congreso
|
01/07/2012 |
|
Fecha fin congreso
|
05/07/2012 |
|
Desde la página
|
|
|
Hasta la página
|
|
|
Título de las actas
|
Acta Horticulturae |