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Genetic stability in mint (Mentha xpiperita) cryopreserved apices: can the cryopreservation technique, regeneration medium composition and genotype affect the final result?

Research Areas
  • Crop production

Aromatic plants have experimented in the last years an increase attention and importance as crops due to their demand by consumers for culinary, medicinal and other applications. In this context, search of wild genotypes, breeding programmes and genotype conservation are some of the tasks involved in the development of the production of these species. One of the most reliable methods for long-term conservation is cryopreservation, mainly due to its capability to guarantee the genetic stability of the preserved material during the process. However, the stresses suffered due to the treatments applied together with in vitro culture required for the regeneration of the plant material may result in some degree of genetic instability (Harding, 2004; Martín and González-Benito, 2005; Martín et al., 2011a). Mint (Mentha ?piperita) is an unfertile hybrid with clonal propagation, this makes cryopreservation a very useful conservation technique, avoiding the risks of field collections and time consuming and labour costs of in vitro conservation. However, cryopreservation conditions must be controlled to guarantee an adequate regeneration rate together with a high genetic stability. Two cryopreservation techniques (droplet-vitrification vs. encapsulation dehydration) were compared with the mint genotype MEN 186. The genetic analysis, using RAPD markers, revealed a higher variation in the regenerated plants from the encapsulation-dehydration protocol (Martín et al. 2011b). In this work the genetic and epigenetic stability analyses of mint apices from a different genotype, MEN 198, cryopreserved under the same techniques and regenerated using three different media composition were carried out. Comparisons of the different treatments on the two mint genotypes are evaluated in order to obtain a protocol of cryopreservation technique and regeneration medium that could optimized the quality of the regenerated plants.
2nd Symposium on Horticulture in Europe (SHE 2012)
Angers, Francia
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Acta Horticulturae

Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Biodiversidad y conservación de recursos fitogenéticos
  • Departamento: Biología Vegetal