Memorias de investigación
Artículos en revistas:
Colocalization of ?-actinin and synaptopodin in the pyramidal cell axon initial segment
Año:2012
Áreas de investigación
  • Ciencias naturales y ciencias de la salud,
  • Ingenierías
Datos
Descripción
The cisternal organelle that resides in the axon initial segment (AIS) of neocortical and hippocampal pyramidal cells is thought to be involved in regulating the Ca(2+) available to maintain AIS scaffolding proteins, thereby preserving normal AIS structure and function. Through immunocytochemistry and correlative light and electron microscopy, we show here that the actin-binding protein ?-actinin is present in the typical cistenal organelle of rodent pyramidal neurons as well as in a large structure in the AIS of a subpopulation of layer V pyramidal cells that we have called the "giant saccular organelle." Indeed, this localization of ?-actinin in the AIS is dependent on the integrity of the actin cytoskeleton. Moreover, in the cisternal organelle of cultured hippocampal neurons, ?-actinin colocalizes extensively with synaptopodin, a protein that interacts with both actin and ?-actinin, and they appear concomitantly during the development of these neurons. Together, these results indicate that ?-actinin and the actin cytoskeleton are important components of the cisternal organelle that are probably required to stabilize the AIS.
Internacional
Si
JCR del ISI
Si
Título de la revista
Cerebral Cortex
ISSN
1047-3211
Factor de impacto JCR
6,544
Información de impacto
Volumen
22
DOI
10.1093/cercor/bhr251.
Número de revista
7
Desde la página
1648
Hasta la página
1661
Mes
JULIO
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Participantes
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Centro o Instituto I+D+i: Centro de tecnología Biomédica CTB