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Memorias de investigación
Artículos en revistas:
Building functional surfaces for biosensors development
Año:2013
Áreas de investigación
  • Ingenierías
Datos
Descripción
Polyelectrolyte multilayers (PEM) built by layer-by-layer technique have been extensively studied over the last years, resulting in a wide variety of current and potential applications. This technique can be used to construct thin films with different functionalities, or to functionalize surfaces with substantial different properties of those of the underlying substrates. The multilayering process is achieved by the alternate adsorption of oppositely charged polyelectrolytes. In this work we get advantage of the protein resistant property of the Poly(1-lysine)-graft(polyethyleneglycol) to create protein patterns. Proteins can be immobilized on a surface by unspecific physical adsorption, covalent binding or through specific interactions. The first protein used in this work was laccase, a copper-containing redox enzyme that catalyse the oxidation of a broad range of polyphenols and aromatic substrates, coupled to the reduction of O-2 to H2O without need of cofactors. Applications of laccases have been reported in food, pulp, paper, and textile industry, and also in biosensor development. Some uses require the immobilization of the enzyme on solid supports by adsorption, covalent attachment, entrapment, etc, on several substrates. Especially for biosensor development, highly active, stable and reproducible immobilization of laccase is required.
Internacional
Si
JCR del ISI
Si
Título de la revista
Key Engineering Materials
ISSN
1013-9826
Factor de impacto JCR
0,224
Información de impacto
Datos JCR del año 2005
Volumen
543
DOI
10.4028/www.scientific.net/KEM.543.204
Número de revista
Desde la página
204
Hasta la página
207
Mes
SIN MES
Ranking
0
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Carlos Moron Fernandez (UPM)
  • Autor: Alfonso Garcia Garcia (UPM)
  • Autor: Enrique Tremps Guerra (UPM)
  • Autor: Jose Andres Somolinos Sanchez (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Grupo de sensores y actuadores
  • Departamento: Tecnología de la Edificación
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