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Memorias de investigación
Ponencias en congresos:
Cis-, trans-, transcriptional regulation of the Arabidopsis thaliana gene AtTrxo1 and its response upon germination and to salt (Póster galardonado con el primer premio)
Año:2013
Áreas de investigación
  • Biología molecular,
  • Investigación agronómica
Datos
Descripción
Plants contain several genes encoding thioredoxins (Trxs), small proteins involved in redox regulation of many enzymes in different cell compartments. Among them, mitochondrial Trxo has been described to have a response in plants grown under salinity but there is scarce information about its functional role in abiotic stress or its gene regulation. In this work, the transcriptional regulation of the mitochondrial AtTrxo1 gene has been studied for the first time, by identifying functionally relevant cis- elements in its promoter: two conserved motives were found as positive and one as negative regulators. Using them as baits for the screening of an arrayed yeast library containing Arabidopsis Transcription Factors (TF) ORFs, two TFs were selected that are now being validated at the molecular level. We have also studied the response of T-DNA insertion mutant plants for AtTrxo1 to salt stress. The K.O. AtTrxo1 mutants presented several phenotypic changes including the time required to reach 50% germination under salinity, without affecting the final germination percentage.
Internacional
Si
Nombre congreso
11th International POG Conference (Reactive Oxygen and Nitrogen Species in Plants).
Tipo de participación
960
Lugar del congreso
Varsovia (Polonia)
Revisores
Si
ISBN o ISSN
0860-7796
DOI
Fecha inicio congreso
17/07/2013
Fecha fin congreso
19/07/2013
Desde la página
558
Hasta la página
558
Título de las actas
BioTechnologia Volume 94(2) 2013
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Ana María Ortíz Espín (CEBAS-CSIC)
  • Autor: Raquel Iglesias Fernandez (UPM)
  • Autor: I. Martínez- Alcalá (CEBAS-CSIC)
  • Autor: Aingeru Calderón (CEBAS-CSIC)
  • Autor: D. Camejo (CEBAS-CSIC)
  • Autor: Francisca Sevilla (CEBAS-CSIC)
  • Autor: Pilar Carbonero Zalduegui (UPM)
  • Autor: Ana Jiménez (CEBAS-CSIC)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Biotecnología y Genómica de Semillas
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
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