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Memorias de investigación
Ponencias en congresos:
Mitochondrial AtTRXo1: its transcriptional regulation and role under salt-stress (póster)
Año:2013
Áreas de investigación
  • Biología molecular,
  • Investigación agronómica
Datos
Descripción
Thioredoxins (Trxs) are ubiquitous small proteins involved in the reduction of disulfide bonds of other proteins in different cell compartments. Among them, mitochondrial Trxo has been described to have a response in plants grown under salinity but there is scarce information about its functional role in abiotic stress or its gene regulation. In this work, the transcriptional regulation of the mitochondrial AtTrxo1 gene has been studied for the first time, by producing severa! AtTrxo1::GUS transgenic Arabidopsis plants containing conserved domains chosen comparing Brasicacea Trxo1 promoters. Three motifs were found as possible positive regulators and one as negative of AtTrxo1 expression. Using an arrayed yeast library from A. thaliana, a set of transcription factors were shown as prey of AtTrxo1 prometer, and among them, we underlined Bzip9 and AZF2, both strongly related with biotic and abiotic stress, respectively. We have also studied the response of mutant AtTrxo1 plants to salinity, in arder to gain insight on its functional role under stress. For it, a physiological characterization of the mutant and the study on its germination pattern was carried out. Results indicated that the lack of AtTrxo1 produced phenotypic changes and influenced the time to 50% germination under salinity, without affecting the final germination percentage. Results are discussed in relation to Trxo1 expression pattern and its regulation.
Internacional
No
Nombre congreso
VIII REUNIÓN del GRUPO ESPAÑOL DE INVESTIGACIÓN EN RADICALES LIBRES {GEIRLI)
Tipo de participación
960
Lugar del congreso
Valencia
Revisores
Si
ISBN o ISSN
0000-0000
DOI
Fecha inicio congreso
03/06/2013
Fecha fin congreso
05/06/2013
Desde la página
59
Hasta la página
59
Título de las actas
VIII REUNIÓN del GRUPO ESPAÑOL DE INVESTIGACIÓN EN RADICALES LIBRES {GEIRLI)
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Ana María Ortíz Espín (CEBAS-CSIC)
  • Autor: Raquel Iglesias Fernandez (UPM)
  • Autor: Aingeru Calderón (CEBAS-CSIC)
  • Autor: Francisca Sevilla (CEBAS-CSIC)
  • Autor: Pilar Carbonero Zalduegui (UPM)
  • Autor: Ana Jiménez (CEBAS-CSIC)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Biotecnología y Genómica de Semillas
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
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