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Memorias de investigación
Ponencias en congresos:
Characterization of HupE, a nickel transporter for hydrogenase synthesis in the endosymbiotic bacterium Rhizobium leguminosarum bv. viciae.
Áreas de investigación
  • Bacteriología
Rhizobium leguminosarumbv. viciae (Rlv) UPM791 synthesizes a NiFe hydrogenase that allows the recycling of hydrogen produced by nitrogenase. In this bacterium, the synthesis of hydrogenase requires the participation of 18 proteins encoded in the hup/hyp gene cluster hupSLCDE FGHIJKhypABF CDEX located in the symbiotic plasmid. One of the genes of this cluster, hupE , encodes a protein harboring a canonical signal peptide and six potential transmembrane domains. Previous hydrogenase activity and Ni63 transport assays demonstrated that HupE is a nickel transporter that provides this metal for the synthesis of hydrogenase in Rlv UPM791 free-living cells and lentil bacteroids, and led to the identification of two histidine-rich motifs required for HupE functionality (Brito et al., 2010). A similar protein (UreJ) is present in gene clusters involved in urease synthesis in other bacteria. HupE/UreJ constitutes a family of single permeases widely distributed in bacteria. The characterization of kinetic and functional properties of Rlv UPM791 HupE is being carried out through heterologous expression of HupE in a nik -deficient Escherichia coli background. The specificity of this transporter for Ni2+ is being determined in competition assays with other divalent metal ions. A HupE variant lacking the N-terminal periplasmic region of the mature protein has been constructed to ascertain the potential role of this domain in ion scavenging and concentration around the transporter. The relevance of other residues conserved in members of the HupE/UreJ family is being tested through the analysis of site-specific mutants. The results of the on-going work will be presented and discussed.
Nombre congreso
10th International Hydrogenase Conference
Tipo de participación
Lugar del congreso
Szeged, Hungría
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Título de las actas
Book of Abstracts
Esta actividad pertenece a memorias de investigación
  • Autor: Marta Albareda Contreras (UPM)
  • Autor: Marie Andree Mandrand-Berthelot (Universidad de Lyon)
  • Autor: Agnes Rodrigue (Universidad de Lyon)
  • Autor: Tomas-Andres Ruiz Argueso (UPM)
  • Autor: Juan Imperial Ródenas (UPM)
  • Autor: Jose Manuel Palacios Alberti (UPM)
  • Autor: Maria Belen Brito Lopez (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Asociaciones simbióticas planta-microorganismo
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
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