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Descripción
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| The use of protein and/or isoenzyme seed patterns as a whole is not, strictly speaking, an analysis of population genetics. Yet, it constitutes a rapid screening strategy for obtaining and comparing ele ctrophoretic profiles of population samples. It can also be applied to both in situ and ex situ con servation of plant species since a maximum amount of information can be obtained by employ ing relatively little time, cost, and effort. In this case, the electrophoregrams represent the ele ctrophoretic phenotype of the whole population and taxonomic numerical analyses must consider all bands as qualitative characters, with no allelic value. Although this work is focused mainly on checking the utility of this methodology at the level of Brassica montana populations, we have also used as ?outgroups? other 2n=18 species whose genetic relationships have been studied from other points of view to verify whether the results are taxonomically coherent. Seed globulins and six isoenzyme systems of eleven populations of B. montana and other allied taxa belonging to sect. Brassica were analyzed. The globulin and isoenzyme banding patterns were analyzed using the UPGMA algorithm. Results showed that a correlation exists between the populations of B. montana and their geographical distribution in the NE of Spain. The relationship between this taxon and its studied relatives is in agreement with the recognized species taxonomy. The results show that the potential use of isoenzymatic profiles of the accessions as a whole constitute an appro priate strategy for sample characterization and identification of new bank samples of pre-existing populations in a germplasm bank. | |
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Internacional
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Si |
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JCR del ISI
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Si |
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Título de la revista
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Journal of Biological Research-Thessaloniki |
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ISSN
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1790-045X |
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Factor de impacto JCR
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0,618 |
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Información de impacto
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Datos JCR del año 2012 |
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Volumen
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20 |
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DOI
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Número de revista
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Desde la página
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243 |
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Hasta la página
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258 |
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Mes
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JUNIO |
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Ranking
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