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Descripción
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| The 3' end polyadenylation of pre-mRNAs is a two-step process. First, pre-mRNAs are cleaved at their 3' end. The second step involves the addition of the polyA tail by RNA polymerases. Presence of multiple potential 3' end cleavage sites is common in eukaryotic genes, and the selection of the right site is regulated during development and in response to cellular cues. This mechanism of alternative (or non-canonical) polyadenylation generates mRNA isoforms with different exon content or 3' UTR lengths and regulates the presence of cis elements in the mRNA. Proteins involved in alternative polyadenylation (APA) include Cleavage Factor I in metazoans (CFIm), Hrp1 in yeast and Rbp35 in filamentous fungi. The cis elements present in the 3' UTRs such as miRNA target sites modulate gene expression by affecting cytoplasmic polyadenylation, subcellular localization, stability, translation and/or decay of the mRNA. Therefore, the selection of a proper 3' end cleavage site represents an important step of regulation of gene expression. Using Direct RNA Sequencing (DRS), we are carrying out in the rice blast fungus Magnaporthe oryzae a comprehensive map of genome wide polyadenylation sites and quantifying their usage under different nutritional conditions (rich and minimal media, carbon and nitrogen starvation) in the wild type and the Drbp35 mutant. Results of these polyadenylation maps will be presented, including candidate APA targets, sequence motifs present in long 3' UTRs, Rbp35-dependent mRNA isoforms, and conservation of significant mRNA isoforms in other filamentous fungi. | |
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Internacional
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Si |
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Nombre congreso
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27th Fungal Genetics Conference |
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Tipo de participación
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960 |
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Lugar del congreso
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Pacific Grove (Canada) |
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Revisores
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Si |
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ISBN o ISSN
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0000-0000 |
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DOI
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Fecha inicio congreso
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12/03/2013 |
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Fecha fin congreso
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17/03/2013 |
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Desde la página
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53 |
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Hasta la página
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54 |
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Título de las actas
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Fungal Genetics Program Book |