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Memorias de investigación
Artículos en revistas:
CRYOPRESERVATION OF THYMUS LOTOCEPHALUS SHOOT TIPS AND ASSESSMENT OF GENETIC STABILITY
Año:2014
Áreas de investigación
  • Biodiversidad
Datos
Descripción
BACKGROUND: Thymus lotocephalus is a rare endemic species from the Algarve, Portugal, and is legally protected by Portuguese and European legislation. OBJECTIVE: The aim is to develop a cryopreservation protocol for T. lotocephalus shoot tips, as an alternative approach for the long-term conservation of this species. METHODS: Several methods (droplet-vitrification, vitrification and encapsulation-dehydration) were tested. Conditions regarding the subculture period, cold-hardening and preculture were optimized. Cryopreserved shoot tips were also assessed for their genetic stability using RAPD markers. RESULTS: Droplet-vitrification presented the best results. The best regrowth of cryopreserved shoot tips obtained eight weeks after rewarming was 67%. This was accomplished with four weeks subculture period of in vitro-donor plants at 25ºC, preculture of excised shoot tips for one day on MS medium containing 0.3 M sucrose, treatment in PVS2 for 60 min, and MS supplemented with 0.2 mg l-1 zeatin as recovery medium. The assessment using RAPD markers observed variation at a low frequency and shoots regenerated from cryopreserved apices showed normal development compared to the regular in vitro-grown shoots. CONCLUSION: Droplet-vitrification is thus a viable method for the cryopreservation of T. lotocephalus shoot tips.
Internacional
Si
JCR del ISI
Si
Título de la revista
CryoLetters
ISSN
0143-2044
Factor de impacto JCR
Información de impacto
Volumen
35
DOI
Número de revista
Desde la página
119
Hasta la página
128
Mes
SIN MES
Ranking
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Participantes
  • Autor: Natacha Coelho (University of Algarve)
  • Autor: M. Elena Gonzalez Benito (UPM)
  • Autor: Maria Carmen Martin Fernandez (UPM)
  • Autor: Anabela Romano (University of Algarve)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Departamento: Biotecnología - Biología Vegetal
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