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Memorias de investigación
Artículos en revistas:
APPROACHES FOR THE CRYOPRESERVATION OF Plantago algarbiensis, A RARE ENDEMIC SPECIES OF THE ALGARVE
Año:2014
Áreas de investigación
  • Biodiversidad
Datos
Descripción
BACKGROUND: Plantago algarbiensis is an endangered endemic species from the Algarve, Portugal. OBJECTIVE: The main goal of this study was to investigate the viability of cryopreservation procedures in the conservation of seeds and nodal explants from this species. MATERIALS AND METHODS: Seeds were directly immersed in liquid nitrogen (LN) for 30 days. Two methods were tested for the cryopreservation of nodal explants, namely droplet-vitrification and encapsulation-dehydration. For both methods, nodal segments were precultured on Murashige and Skoog (MS) medium and recovered on MS supplemented with 0.2 mg l-1 6-benzyladenine (BA), after freezing. RESULTS: After 30 days in LN, the germination capacity of seeds was not affected. The regrowth percentages of cryopreserved nodal segments were approximately 60%. With the droplet-vitrification method, a regrowth percentage of 60.0 ± 15.2% was obtained after 120 min exposure to PVS2 (plant vitrification solution 2) and with encapsulation-dehydration method the highest percentage, 63.3 ± 9.6%, was achieved after 3 h desiccation. CONCLUSION: Seed cryopreservation and cryopreservation of nodal segments by droplet-vitrification and encapsulation-dehydration are therefore effective approaches for the conservation of P. algarbiensis
Internacional
Si
JCR del ISI
Si
Título de la revista
Cryoletters
ISSN
0143-2044
Factor de impacto JCR
0,64
Información de impacto
Datos JCR del año 2013
Volumen
35
DOI
Número de revista
Desde la página
521
Hasta la página
529
Mes
SIN MES
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Natacha Coelho (University of Algarve)
  • Autor: M. Elena Gonzalez Benito (UPM)
  • Autor: Anabela Romano (University of Algarve)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Biodiversidad y conservación de recursos fitogenéticos
  • Departamento: Biotecnología - Biología Vegetal
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