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Abstract
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| Trees from temperate regions have developed an adaptive mechanism called winter dormancy aimed to survive the extreme climate changes that take place during this season. The circadian clock is involved in the transduction of daylenght and temperature signals that regulate the winter dormancy. In chestnut in response to cold, circadian clock genes lose their rhythmic expression and become constitutively activated (1, 2). This molecular response to cold also occurs in Arabidopsis but only when light/dark cues are absent (3). We are interested in understanding how this specific molecular response is controlled in order to shed light on the biological meaning of the cold-induced constitutive activation of circadian clock genes in perennials. Our hypothesis is that cold-induceddisruption of circadian clock genes in woody plants is due to a transcriptional control operating above these genes. First of all we wanted to check whether poplar, the model tree specie, showed this alteration as well as chestnut. Once we had confirmed the disruption in natural conditions, we cloned the promoter of a circadian clock gene from Populus tremula x alba fused to the luciferase reporter. Poplar plants stably transformed with this construct were used to perform luminescence assays under different photoperiods and temperature conditions. Our results indicate that the selected promoter fragment contains the elements both to keep the circadian rhythm at 21ºC and to drive the cold-induced disruption of circadian clock gene expression. | |
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International
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No |
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Congress
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XII Reunión de Biología Molecular de Plantas |
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960 |
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Place
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Reviewers
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Si |
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ISBN/ISSN
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0000-0000 |
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Start Date
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11/06/2014 |
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End Date
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13/06/2014 |
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From page
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232 |
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To page
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232 |
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XII Reunión de Biología Molecular de Plantas |