Observatorio de I+D+i UPM

Memorias de investigación
Artículos en revistas:
The role of N-glycosilation in kiwi allergy
Año:2014
Áreas de investigación
  • Mecanismos moleculares de enfermedad
Datos
Descripción
The physical, biochemical, and immunological characteristics of plant allergens have been widely studied, but no definite conclusion has been reached about what actually makes a protein an allergen. In this sense, N-glycosylation is an exclusive characteristic of plant allergens not present in mammals and it could be implied in allergenic sensitization. With this aim, we evaluated and compared the allergenic activity of the protein fraction and the N-glycan fraction of the thaumatin-like protein and the main kiwi allergen, Act d 2. The natural allergen, Act d 2, was deglycosylated by trifluoromethanesulfonic acid treatment; the N-glycan fraction was obtained by extended treatment with proteinase K. N-glycan- and protein- fractions were recognized by specific IgE of kiwi-allergic patients. By contrast, the sugar moiety showed a reduced capacity to activate basophils and T cells, but not dendritic cells derived from patients? monocytes. Related to this, the production of cytokines such as IL6 and IL10 was increased by the incubation of dendritic cells with sugar moiety. Thus, the sugar moiety plays a significant role in sensitization, inducing the activation of antigen-presenting cells, but it is the protein fraction that is responsible for the allergic reactions.
Internacional
No
JCR del ISI
No
Título de la revista
Food Science & Nutrition
ISSN
2048-7177
Factor de impacto JCR
Información de impacto
Volumen
3
DOI
Número de revista
Desde la página
260
Hasta la página
271
Mes
MAYO
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Maria Garrido Arandia (UPM)
  • Autor: Cristina Gomez Casado (UPM)
  • Autor: Amaya Murua Garcia (UPM)
  • Autor: Arantxa Palacin
  • Autor: Leticia Tordesillas (UPM)
  • Autor: Natalia Blanca-López
  • Autor: Tania Ramos
  • Autor: Gabriela Canto
  • Autor: Javier Cuesta-Herranz
  • Autor: Rosa Sánchez-Monge (UPM)
  • Autor: Luis Fernandez Pacios (UPM)
  • Autor: Araceli Diaz Perales (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Biotecnología Vegetal
  • Departamento: Sistemas y Recursos Naturales
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
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