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Memorias de investigación
Artículos en revistas:
Cryopreservation of shoot tips from the endangered endemic species Tuberaria major
Año:2014
Áreas de investigación
  • Biodiversidad
Datos
Descripción
Tuberaria major is an endangered endemic species from the Algarve, in the south of Portugal. We investigated two techniques for the cryopreservation of T. major shoot tips, namely vitrification and encapsulationdehydration. Before the cryopreservation trials, shoot tips were precultured for 1 day on liquid Murashige and Skoog (MS) medium containing 0.3 M sucrose. For the vitrification method, shoots tips were exposed for 0, 30, 60, 90 and 120 min to plant vitrification solution 2 (PVS2). As for the encapsulation-dehydration method, shoot tips were dried inside a laminar air flow cabinet for 0, 1, 2, 3, 4, 5 and 6 h at room temperature. The highest regrowth percentages were approximately 60 and 67 % for vitrification and encapsulation-dehydration, respectively. The best times were 60 min exposure to PVS2 for vitrification and 3 h desiccation for encapsulation-dehydration. Though these are preliminary results, the use of the cryopreservation techniques tested here proved to be an important asset in the conservation of this endangered species and will complement the conservation strategies previously developed.
Internacional
Si
JCR del ISI
Si
Título de la revista
Acta Physiologiae Plantarum
ISSN
0137-5881
Factor de impacto JCR
1,524
Información de impacto
Volumen
36
DOI
Número de revista
Desde la página
3333
Hasta la página
3336
Mes
SIN MES
Ranking
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Participantes
  • Autor: Natacha Coelho (University of Algerve)
  • Autor: M. Elena Gonzalez Benito (UPM)
  • Autor: Anabela Romano (University of Algerve)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Biodiversidad y conservación de recursos fitogenéticos
  • Departamento: Biotecnología - Biología Vegetal
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