Observatorio de I+D+i UPM

Memorias de investigación
Artículos en revistas:
Regeneration in min (Mentha x piperita) cryopreserved apices: can the cryopreservation technique, regeneration medium composition and genotype affect the final result?
Año:2015
Áreas de investigación
  • Biodiversidad,
  • Producción vegetal
Datos
Descripción
One of the most reliable methods for long-term conservation is cryopreservation, mainly due to its capability to guarantee the genetic stability of the preserved material. During the development of cryopreservation protocols, the composition of the growth recovery medium is of vital importance in order to obtain fully viable structures without callus formation. Two cryopreservation techniques (droplet-vitrification and encapsulation-dehydration) and three different recovery media were compared with shoot apices of two mint genotypes (?MEN186? and ?MEN198?). The medium was supplemented with: 1) 0.5 mgL-1 6-benzylaminopurine; 2) 0.5 mgL-1 6-dimethylallylamino-purine + 0.1 mgL-1 ?-naphthalene acetic acid; or 3) 0.5 mgL-1 6-benzylaminopurine + 0.1 mgL-1 ?-naphthalene acetic acid. Survival and recovery (shoot growth) were studied after a 4-week recovery period. Although the recovery media did not influence survival in any of the two genotypes or protocols, the recovery percentage observed was affected by the growth regulators. When the encapsulation-dehydration protocol was employed this influence was detected even in control apices (no subjected to cooling in liquid nitrogen), while with the droplet-vitrification method recovery medium had an effect only on cryopreserved apices. In all these cases, the medium without auxin (only with cytokinin) resulted in higher recovery rates, i.e. organized growth was favoured.
Internacional
Si
JCR del ISI
No
Título de la revista
Acta Horticulturae
ISSN
0567-7572
Factor de impacto JCR
Información de impacto
Volumen
DOI
Número de revista
Desde la página
777
Hasta la página
782
Mes
SIN MES
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: M. Elena Gonzalez Benito (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: PLANGER Germoplasma Vegetal
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