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Memorias de investigación
Artículos en revistas:
Medicago truncatula Natural Resistance Associated Macrophage Protein1 is required for iron uptake by rhizobia infected nodule cells
Año:2015
Áreas de investigación
  • Ciencias naturales y ciencias de la salud
Datos
Descripción
Iron is critical for symbiotic nitrogen fixation (SNF) as a key component ofmultiple ferroproteins involved in this biological process. In the model legume Medicago truncatula, iron is delivered by the vasculature to the infection/maturation zone (zone II) of the nodule, where it is released to the apoplast. From there, plasma membrane iron transporters move it into rhizobia-containing cells, where iron is used as the cofactor of multiple plant and rhizobial proteins (e.g. plant leghemoglobin and bacterial nitrogenase). MtNramp1 (Medtr3g088460) is the M. truncatula Natural Resistance-Associated Macrophage Protein family member, with the highest expression levels in roots and nodules. Immunolocalization studies indicate that MtNramp1 is mainly targeted to the plasma membrane. A loss-of-function nramp1 mutant exhibited reduced growth compared with the wild type under symbiotic conditions, but not when fertilized with mineral nitrogen. Nitrogenase activity was low in the mutant, whereas exogenous iron and expression of wild-type MtNramp1 in mutant nodules increased nitrogen fixation to normal levels. These data are consistent with a model in which MtNramp1 is the main transporter responsible for apoplastic iron uptake by rhizobia-infected cells in zone II.
Internacional
Si
JCR del ISI
Si
Título de la revista
Plant Physiol
ISSN
0032-0889
Factor de impacto JCR
6,841
Información de impacto
Volumen
168
DOI
Número de revista
Desde la página
258
Hasta la página
272
Mes
SIN MES
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Manuel Tejada Jiménez (UPM)
  • Autor: M del Rosario Castro Rodriguez (UPM)
  • Autor: Igor Kryvoruchko (Noble Foundation)
  • Autor: M. Mercedes Lucas (CSIC)
  • Autor: Michael Udvardi (Noble Foundation)
  • Autor: Juan Imperial Ródenas (UPM)
  • Autor: Manuel Gonzalez Guerrero (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL
  • Departamento: Biotecnología - Biología Vegetal
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