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Memorias de investigación
Capítulo de libro:
Functional genomics of symbiotic nitrogen fixation in legumes with a focus on transcription factors and membrane transporters
Año:2015
Áreas de investigación
  • Ciencias naturales y ciencias de la salud
Datos
Descripción
Transcription factors (TFs), transcription regulators (TR), and membrane transporters are major determinants of nodule development and function. TFs and TRs control genetic reprogramming, and transporters facilitate exchange of nutrients between plant and rhizobia among other things. Here, we describe the strategy of reverse genetic screening in Medicago truncatula for genes from these two functional classes. We employed well-established transcriptomic resources (Medicago gene expression atlas), a spatially resolved nodule transcriptomic data set, and the tobacco retrotransposon Tnt1 insertion mutant population for systematic selection and functional analysis of candidate genes. We also re-annotated 417 nodule-induced TF and TR genes according to IMGAG3.5v4, which is the latest release of the Medicago genome. In total, we have targeted 21 nodule-enhanced TF and TR genes, and 20 nodule-enhanced transporter genes for characterization via insertional mutagenesis. In this chapter, we emphasize the importance of TFs, TRs, and transport proteins for symbiotic nitrogen fixation (SNF) and discuss the use of the Medicago Tnt1 mutant population for studies of gene function.
Internacional
Si
DOI
10.1002/9781119053095.ch82
Edición del Libro
Editorial del Libro
2015 John Wiley & Sons, Inc
ISBN
9781119053095
Serie
Título del Libro
Biological Nitrogen Fixation
Desde página
823
Hasta página
836
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Senjuti Sinharoy (Noble Foundation)
  • Autor: Igor Kryvoruchko (Noble Foundation)
  • Autor: Catalina I. Pislariu (Noble Foundation)
  • Autor: Manuel Gonzalez Guerrero (UPM)
  • Autor: Vagner A. Benedito (West Virginia University)
  • Autor: Michael K. Udvardi (Noble Foundation)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
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