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Selection of specific Rhizobium leguminsoarum genotypes by different Fabeae legume host: A Pool-Seq mesocosm study

Research Areas
  • Natural sciences and health sciences

Rhizobium leguminosarum bv viciae can establish effective symbioses with members of the Fabeae legume tribe (Pisum, Lathryrus, Lens and Vicia). Previous studies have suggested that different Fabeae select specific genotypes of rhizobia from those available in soil. We extended these observations at the genomic level by applying a Pool-Seq analysis to isolates selected from a soil population by pea, lentil, fava and vetch plants, and showed that plant- selected sub-populations differ at the single nucleotide polymorphism level. The nature and extent of plant-specific genotypic preference were further studied by performing controlled mesocosm assays. Sterilized seedlings were planted in soil:vermiculite 1:1, and 21-day roots were collected, sterilized superficially, homogenized and centrifuged at low speed. This supernatant was used as inoculant for the next cycle of selection in the same soil with new seedlings. After 5 cycles, roots were collected, and rhizobia (25 from each host) isolated from nodules. Each of the isolates was grown separately, all cultures from the same host were pooled, and their pooled DNA was isolated and subjected to Pool-Seq analysis. Sequence reads were aligned against the R. leguminosarum bv viciae 3841 reference genome and both coverage and polymorphism analyses were performed for specific regions. No genotypic selection by the host was observed in the rDNA region, whereas increased genotypic selection was very clear along the mesocosm experiment for the nod cluster, but only in the pea and vetch subpopulations. In contrast, no further polymorphism profile changes were observed in the nod region for the fava and lentil subpopulations after the first round of plant host selection. These results suggest the existence of differences in plant host selection of rhizobial genotypes along the mesocosm experiment for different hosts and genomic regions.
Rhizosphere 4
Maastricht (Holanda)
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Rhizosphere 4
  • Autor: Beatriz Jorrin Rubio UPM
  • Autor: Amalia Soenens Martínez de Murguia UPM
  • Autor: Juan Imperial Ródenas UPM

Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP