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Memorias de investigación
Ponencias en congresos:
Characterisation of medium-term selection of Rhizobium leguminosarum bv. viciae populations by different plant hosts
Año:2015
Áreas de investigación
  • Ciencias naturales y ciencias de la salud
Datos
Descripción
Rhizobium leguminosarum bv viciae is a nitrogen-fixing soil bacterium able to establish specific root-nodule symbioses with legumes of four different genera: Pisum, Vicia, Lens and Lathyrus. Available evidence suggests that although Rhizobium leguminosarum bv viciae strains are able to nodulate the four plant genera, plant hosts select specific rhizobial genotypes from those present in the soil. This has been shown previously in our laboratory at the genomic level following a population genomics approach (Pool-Seq). Pool genomic sequences from 100 isolates from each of four plant species: P. sativum, L. culinaris, V. faba and V. sativa show different specific profiles at the single nucleotide polymorphism (SNP) level for relevant genes. In this work we characterised the extent of plant host selection on Rhizobium leguminosarum bv viciae genotypes both in nodules and in the soil. For that purpose we proceed to do a mesocosm experiment, where we started with a well- characterised soil population, applied plant host selection and those rhizobia selected by the different legume hosts: P. sativum, L. culinaris, V. faba and V. sativa were used as the inoculum for the next plant growth. This was done for 5 cycles. Direct soil and nodule individual strains from each of these mesocosm studies have been isolated and initially tested for specific rhizobial genes (glnII and fnrN) and symbiotic genes (nodC and nifH). The selected populations were further characterised by means of Sanger sequencing of both the rpoB phylogenetic marker gene and the symbiotic genes nodC and nifH. The distribution and composition of the different rhizobial populations showed changes for each legume host during the mesocosm study. We hypothesize that these changes mimic those occurring under real field conditions and should help reveal the underlying bases of rhizobial genotype selection by the plant host.
Internacional
Si
Nombre congreso
Rhizosphere 4
Tipo de participación
970
Lugar del congreso
Maastricht (Holanda)
Revisores
Si
ISBN o ISSN
0000000000
DOI
Fecha inicio congreso
21/06/2015
Fecha fin congreso
25/06/2015
Desde la página
295
Hasta la página
296
Título de las actas
Rhizosphere 4
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Amalia Soenens Martínez de Murguia (UPM)
  • Autor: Beatriz Jorrin Rubio (UPM)
  • Autor: Juan Imperial Ródenas (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
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