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Descripción
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| The purpose of this study was to generate an in vitro wound healing model based on stratified human corneal-limbal epithelial (HCLE) cells that resembles the native corneal epithelium. Immortalized HCLE cells were grown to confluence in serum-free medium and switched to 10% serum to promote differentiation and stratification. Afterwards, a mechanical injury with a trephine, combined with epithelial debridement, was performed. Cells were grown in culture media DMEM/F12 supplemented with EGF, with or without serum. The effect of serum on the wound healing response was evaluated using phase-contrast microscopy and a barrier function (rose bengal uptake) assay. | |
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Internacional
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Si |
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Nombre congreso
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6th EuCornea Congress Satellite Meetings |
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Tipo de participación
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960 |
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Lugar del congreso
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Barcelona |
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Revisores
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Si |
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ISBN o ISSN
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1393-8983 |
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DOI
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Fecha inicio congreso
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04/04/2015 |
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Fecha fin congreso
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05/09/2015 |
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Desde la página
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32 |
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Hasta la página
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40 |
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Título de las actas
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Proceeding of Congress |