Memorias de investigación
Ponencias en congresos:
Role of poplar DNA demethylases in controlling winter dormancy
Año:2015
Áreas de investigación
  • Ciencias naturales y ciencias de la salud,
  • Biología molecular, celular y genética,
  • Ciencias biológicas,
  • Biología,
  • Biología molecular,
  • Genómica,
  • Genética de plantas,
  • Ingeniería biológica,
  • Ingeniería genética,
  • Organismos modificados genéticamente,
  • Biología vegetal, animal y ecología
Datos
Descripción
Trees have developed the mechanism of winter dormancy to survive the harsh conditions of winter in temperate and cold regions. It determines the geographical distribution of tree species and the long of the growing period. Epigenetic control of winter dormancy in woody plants is barely known. 5-methyl cytosine (5mC) is an important epigenetic mark that regulates gene expression in animals and plants. The profile of DNA methylation changes in the transition of developmental stages in plants such as chestnut bud set and burst, flowering in azalea, aging in pine trees among other. So far, the enzymes involved in the modification of the methylome and the molecular mechanism behind the control over those development processes remain to be understood. Our previous results showed higher DNA methylation and less acetylated Lys 8 of histone H4 global levels in poplar stem during winter dormancy compared to active growing season. Analysis of the 5-methyl cytosine levels by the application of the immunofluorescence-based method set up in our lab showed that global DNA methylation decrease significantly when trees are near to restore the growing season after the winter dormancy. We have identified two poplar homologs to Arabidopsis DME gene: PtaDML8/PtaDML10. DME protein promotes global DNA demethylation along the genome during the endosperm development. Our RT-PCR analyses indicate that the expression of PtaDML8/PtaDML10 genes increases significantly when trees are near to restart growing after the winter. Functional characterization showed that PtaDML8/PtaDML10 knockdown plants have a delayed in resuming of growth after dormancy. Taken together, we hypothesize that an active control of the 5mC DNA methylation play a key role in winter dormancy and that 5mC demethylases would be crucial for poplar dormant-growth transition
Internacional
No
Nombre congreso
XXI REUNIÓN DE LA SOCIEDAD ESPAÑOLA DE FISIOLOGÍA VEGETAL
Tipo de participación
970
Lugar del congreso
Toledo, España
Revisores
Si
ISBN o ISSN
978-84-606-8883-9
DOI
Fecha inicio congreso
14/06/2015
Fecha fin congreso
17/06/2015
Desde la página
351
Hasta la página
351
Título de las actas
Role of poplar DNA demethylases in controlling winter dormancy
Esta actividad pertenece a memorias de investigación
Participantes
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Biotecnología Vegetal
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal