Memorias de investigación
Communications at congresses:
Towards understanding RAV1 transcriptional network during the growth-dormancy cycle in poplar
Year:2015
Research Areas
  • Natural sciences and health sciences,
  • Earth sciences,
  • Molecular, cellular and genetic biology,
  • Biological sciences,
  • Biology,
  • Molecular biology,
  • Molecular genetics,
  • Plant genetics,
  • Biological engineering,
  • Genetic engineering
Information
Abstract
As plants are sessile organisms, the decision to grow or to stop growing is fundamental for their survival. To survive to the harsh condition of the winter, temperate and boreal trees undergo a self-protective developmental reprograming known as winter dormancy. Its mechanism involves an intricate interplay between endogenous growth regulators and the winter external signal, such as shortening of photoperiod and cold temperatures. Currently, only few factors including circadian and developmental transcription factors and hormonal signaling molecules have been shown to modulate the extension of the dormancy period in woody plants. In our laboratory, the circadian controlled transcription factor RAV promotes sylleptic branching and reduces dormancy length when RAV is ectopic overexpressed in poplar (1). Comparative mRNA profiling of RAV overexpressing versus wild type revealed a transcriptional rearrangement happens as effect of constitutive high level of RAV, giving rise to phenology. However, to define RAV mode of action as a transcriptional regulator and how RAV activity trigger a modification of dormancy-growth cycle, its primary targets genes should be investigated. At this particular, the translational fusion of a given transcription factor to the rat glucorticoid receptor domain (GR) have been widely used in Arabidopsis and recently in poplar (2). In this work we show the standardization of a methodology required to identify primary target genes for a transcription factor in poplar. This will be useful to future exploration of RAV1 gene network and its mode of action in spatio-temporal studies at tissue level during growth-dormancy cycle in poplar.
International
No
Congress
XXI REUNIÓN DE LA SOCIEDAD ESPAÑOLA DE FISIOLOGÍA VEGETAL
970
Place
Toledo, España
Reviewers
Si
ISBN/ISSN
978-84-606-8883-9
Start Date
14/06/2015
End Date
17/05/2016
From page
356
To page
356
Towards understanding RAV1 transcriptional network during the growth-dormancy cycle in poplar
Participants
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Biotecnología Vegetal
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal