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Memorias de investigación
Artículos en revistas:
A Recombinant Sal k 1 Isoform as an Alternative to the Polymorphic Allergen from Salsola kali Pollen for Allergy Diagnosis
Año:2015
Áreas de investigación
  • Ciencias naturales y ciencias de la salud,
  • Biología molecular, celular y genética,
  • Ciencias biológicas,
  • Biología,
  • Ingeniería biológica,
  • Biología vegetal, animal y ecología
Datos
Descripción
BACKGROUND: The incidence of Amaranthaceae pollen allergy has increased due to the desertification occurring in many countries. In some regions of Spain, Salsola kali is the main cause of pollinosis, at almost the same level as olive and grass pollen. Sal k 1 - the sensitization marker of S. kali pollinosis - is used in clinical diagnosis, but is purified at a low yield from pollen. We aimed to produce a recombinant (r)Sal k 1 able to span the structural and immunological properties of the natural isoforms from pollen, and validate its potential use for diagnosis. METHODS: Specific cDNA was amplified by PCR, cloned into the pET41b vector and used to transform BL21 (DE3) Escherichia coli cells. Immunoblotting, ELISA, basophil activation and skin-prick tests were used to validate the recombinant protein against Sal k 1 isolated from pollen. Sera and blood cells from S. kali pollen-sensitized patients and specific monoclonal and polyclonal antisera were used. RESULTS: rSal k 1 was produced in bacteria with a yield of 7.5 mg/l of cell culture. The protein was purified to homogeneity and structural and immunologically validated against the natural form. rSal k 1 exhibited a higher IgE cross-reactivity with plant-derived food extracts such as peanut, almond or tomato than with pollen sources such as Platanus acerifolia and Oleaceae members. CONCLUSIONS: rSal k 1 expressed in bacteria retains intact structural and immunological properties in comparison to the pollen-derived allergen. It spans the immunological properties of most of the isoforms found in pollen, and it might substitute natural Sal k 1 in clinical diagnosis.
Internacional
Si
JCR del ISI
Si
Título de la revista
International Archives of Allergy And Immunology
ISSN
1018-2438
Factor de impacto JCR
2,433
Información de impacto
Volumen
167
DOI
Número de revista
Desde la página
83
Hasta la página
93
Mes
SIN MES
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Salvador Mas (Departamento de Bioquímica y Biología Molecular I, Universidad Complutense de Madrid,)
  • Autor: Patrice Boissy (ALK-Abelló S.A)
  • Autor: Rafael I Monsalve (ALK-Abelló S.A)
  • Autor: Javier Cuesta-Herranz (Hospital Fundación Jiménez Díaz, Madrid)
  • Autor: Araceli Diaz Perales (UPM)
  • Autor: Javier Fernández (Departamento Medicina Clínica, Universidad Miguel Hernández de Elche, Alicante , and)
  • Autor: Carlos Colás (Hospital Clínico Universitario `Lozano Blesa¿, Zaragoza , Spain)
  • Autor: Rosalía Rodríguez (Departamento de Bioquímica y Biología Molecular I, Universidad Complutense de Madrid)
  • Autor: Rodrigo Barderas (Departamento de Bioquímica y Biología Molecular I, Universidad Complutense de Madrid)
  • Autor: Mayte Villalba (Departamento de Bioquímica y Biología Molecular I, Universidad Complutense de Madrid)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Biotecnología Vegetal
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
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