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Abstract
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| Four accessions of rocket [Eruca vesicaria (L.) Cav.] of wild origin held at the Universidad Politécnica de Madrid germplasm bank were regenerated in isolated cages with bumblebees as pollinators. The genetic diversity of parental (P) and regenerated (R) populations was studied by means of molecular markers: RAPD and two Brassica rapa SSR loci. From RAPD markers, UPGMA cluster analysis was performed; diversity was estimated using Shannon?s index and AMOVA. Differences in marker frequency between P and R populations of each accession were analysed through the c2 test. Frequencies of SSR alleles, genetic diversity estimators (FST, FIS) and AMOVA were calculated. The results obtained with RAPD markers were in accordance with those from SSRs and indicate that the regeneration process had only minor effects on the genetic diversity of samples in three out of four studied accessions. Detected changes in the frequency of markers were in most cases negligible and do not seem to constitute either a significant loss of genetic variability or risk of genetic erosion, except when seed dormancy or other factors involving selection are acting, as it might have happened in Ev9107. It has been demonstrated that Brassica rapa SSR markers can be effectively transferred to Eruca vesicaria. | |
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International
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Si |
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JCR
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Si |
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Title
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Seed Science And Technology |
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ISBN
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0251-0952 |
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Impact factor JCR
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0,706 |
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Impact info
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Datos JCR del año 2013 |
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Volume
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44 |
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Journal number
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From page
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1 |
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To page
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15 |
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Month
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SIN MES |
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Ranking
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