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Abstract
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| Nanotechnology is creating high hopes for treating several diseases and different therapies based in the application of nanoparticles are being developed. We have introduced a new technique to control and guide the movement of magnetic micro particle (MMP) in one direction, and it can be used therapeutically in several diseases as: cancer, thrombosis, etc. Our hypothesis is that by means of an alternating gradient magnetic force, the MMPs follow straight line trajectories of a controlled length, in a repetitive way, and MMPs can produce cell death by interacting with cellular structures. In our experimental set-up the force is obtained from a combination of a permanent magnet, which provides a strong magnetic field constant, and an alternating magnetic field provided by a specially designed Helmholtz coils pair. We control the current circulating through the coils for controlling of movement particles. Different size MMPs were incubated with 132 1N1 cells for 24 hours. MMP-cells were then subjected to the magnetic field provided by our engine for 60 min, with a wave 7A square current. Confocal microscopy studies revealed that MMPs are internalized by 132 1N1 cells. Immunofluorescence analysis demonstrated that MMPs are accumulated in lysosomas. MMPs are conducted to the lysosomes of cells where, with controlled MMPs motion cell death occurs. Cell death is more efficiently produced using 2?m MMPs at 1Hz. References [1] Controlled linear movement of nanoparticles in suspension by homogeneous magnetic field gradients, Barcelona, nov 18-20, http://www.nanobiomedconf.com/NBM15/posters.php | |
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International
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Si |
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Congress
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Bionanomed 2016, 7th International Congress, Nanotechnology in Biology & Medicine |
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970 |
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Place
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Reviewers
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Si |
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ISBN/ISSN
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0000-0000 |
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Start Date
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06/04/2016 |
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End Date
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08/04/2016 |
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From page
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0 |
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To page
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0 |
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http://www.bionanomed.at/ |