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Descripción
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| The methodology applied for processing digesta samples before laboratory analysis may affect the analytical results. The aim of this study was to assess the influence of sample processing on the concentration of the volatile fatty acids (VFA) in the cecum of 25-d broilers fed corn- or wheat-based diets. The experimental design was a split-plot, with diet as the main plot and sample processing as the subplot. Each treatment was replicated 8 times (4 broilers each). Cecal samples were obtained by pooling the contents of 2 broilers chosen at random from each replicate. After homogenization, samples were split into 3 aliquots which were allotted to one of the 3 following treatments: CON) immediately frozen and processed without any acidification; FAC) frozen and acidified (1 mL 0.5 N HCl/g) in the laboratory before VFA analyses; and ACF) immediately acidified and frozen. As compared with the ACF, total VFA and acetate concentration decreased by 9.4 and 11.2% with the use of the FAC procedure and by 9.4 and 13.0% with the use of the CON procedure, respectively (P < 0.05). Propionate, butyrate, and isovalerate concentrations were lower (P < 0.05) in the FAC samples than in the ACF and CON samples. Valerate concentration was highest (P < 0.001) in the samples analyzed without any acidification. Main cereal of the diet did not affect total VFA and acetate concentration but corn-fed broilers had higher (P = 0.05) concentrations of propionate, isobutyrate, valerate, and isovalerate, and lower (P < 0.05) of butyrate than wheat-fed broilers. In summary, when the cecal samples were not acidified before being frozen and stored, total VFA and acetate concentrations decreased but the differences detected between diets were similar for all the tested methods. The acidification of the cecal contents at the time of sampling is recommended for improving the accuracy of VFA values. | |
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Internacional
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Si |
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Nombre congreso
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Poultry Science Association |
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Tipo de participación
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970 |
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Lugar del congreso
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Louisville, Kentucky |
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Revisores
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Si |
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ISBN o ISSN
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0032-5791 |
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DOI
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Fecha inicio congreso
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27/07/2015 |
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Fecha fin congreso
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30/07/2015 |
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Desde la página
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168 |
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Hasta la página
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168 |
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Título de las actas
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Effect of the sampling procedure on the determination of volatile fatty acid concentrations in the cecum of broilers fed diets based on corn or wheat. |