Memorias de investigación
Arabidopsis SWC4 is a DNA-binding component of the SWR1 complex and is essential for plant development

Research Areas
  • Natural sciences and health sciences

Deposition of the H2A.Z by the SWR1 complex (SWR1-C) in regulatory regions of specific loci paises genes for transcription. Through the characterization of Arabidopsis mutations in homologs of the yeast SWR1-C, a role for H2A.Z exchange in different plan! developmental processes and in responses to environmental cues has been proposed. However, !he exact composition of the putative plan! SWR1-C subunit and how the complex is selectively recruited to target genes still remains elusive. To fill these knowledge gaps, we identified interaction partners of the SWC6 subunit using a proteomics approach. Among others, we identitied the Arabidopsis homolog of the yeast Swc4/Eaf2. We found that a knock-out allele for Arabidopsis SWC4 is embryo lethal, revealing that this gene, like its yeast orthologue, is essential for development. SWC4 RNAi (swc41) knockdown lines displayed pleiotropic phenotypic alterations in vegetative and reproductive traits including acceleration of flowering . In addition, we will present evidence revealing a role for SWC4 in leaf cell proliferation and expansion, possibly by regulating the progression of the cell cycle. lmportantly, we have demonstrated that Arabidopsis SWC4 is a DNA-binding protein that recognizes AJT-rich regions. These DNA-binding motifs were overrepresented within the promoter sequences of deregulated genes in swc4i plants. We will discuss about the possible molecular mechanisms underlying SWC4 activity in the regulation of H2A.Z deposition
Entity Nationality
Sin nacionalidad
Uppsala, Suecia

Research Group, Departaments and Institutes related
  • Creador: Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP