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Memorias de investigación
Ponencias en congresos:
A Rhizobium leguminosarum bv viciae DNA Region Involved in Host-specific Symbiotic Efficiency
Año:2016
Áreas de investigación
  • Ciencias naturales y ciencias de la salud
Datos
Descripción
The symbiotic nitrogen fixation in legume nodules is the final outcome of a complex interaction between plant and rhizobia. In this interaction, multiple compounds produced either by the plant macrosymbiont (flavonoids, lectins, different kinds of peptides, etc) of by the bacterial microsymbiont (Nod factors, EPS, LPS, etc) participate in a sophisticate molecular dialogue that governs the specificity of the interaction (1). In spite of the number of signals involved, the degree of specificity of the symbiosis is highly variable. In some cases, several legumes can be nodulated by the same rhizobial strain. In the case of Rhizobium leguminosarum bv. viciae, plant species belonging to four different genera (Pisum, Lens, Lathyrus, and Vicia) are permissive for the establishment of efficient nitrogen fixation in nodules. The basis for the ability of this bacterium to interact with different legumes is not fully understood at molecular level. Analysis of a set of randomly generated mutants of R. leguminosarum bv viciae UPM1137 allowed the identification of a mutant impaired on its symbiotic association with pea, inducing only white, ineffective nodules with this host. Interestingly, the mutant retains the wild-type ability to effectively nodulate lentil plants, a phenotype that we designate as Host-specific symbiotic efficiency (Hse). Additionally, the mutant is impaired in the growth in rich media such as TY. Complementation of the mutant with a R. leguminosarum bv. viciae gene bank has allowed the identification of a 3-kb DNA region able to restore the Hse phenotype. The role of the genes present in the hse region is currently being analysed, and the results of this analysis will be presented in the communication.
Internacional
Si
Nombre congreso
12th European Nitrogen Fixation Conference
Tipo de participación
970
Lugar del congreso
Budapest
Revisores
Si
ISBN o ISSN
978-615-5270-26-0
DOI
Fecha inicio congreso
25/08/2016
Fecha fin congreso
28/08/2016
Desde la página
250
Hasta la página
250
Título de las actas
978-615-5270-26-0
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Alejandro Sanz (UPM)
  • Autor: Lu Jiangkun (UPM)
  • Autor: David Ricardo Duran Wendt (UPM)
  • Autor: Laura Rubio Sanz (UPM)
  • Autor: Jose Manuel Palacios Alberti (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
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