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Descripción
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| The extreme sensitivity of nitrogenase towards oxygen stands as a major barrier to engineer biological nitrogen fixation into cereal crops by direct nif gene transfer (1, 2). Here, we use yeast as a model of eukaryotic cell and show that aerobically grown cells express active nitrogenase Fe protein when the NifH polypeptide is targeted to the mitochondrial matrix together with the NifM maturase. Co-expression of NifH and NifM with nif-specific Fe-S cluster biosynthetic proteins NifU and NifS is not required for Fe protein activity, demonstrating NifH ability to incorporate endogenous mitochondrial Fe-S clusters. In contrast, expression of active Fe protein in the cytosol requires both anoxic growth conditions and co-expression of NifH and NifM with NifU and NifS. Our results show the convenience of using mitochondria to host nitrogenase components, thus providing instrumental technology for the grand challenge of engineering N2-fixing cereals (3). | |
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Internacional
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Si |
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Nombre congreso
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12th European Nitrogen Fixation Conference |
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Tipo de participación
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970 |
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Lugar del congreso
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Budapest |
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Revisores
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Si |
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ISBN o ISSN
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0000000000 |
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DOI
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Fecha inicio congreso
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25/08/2016 |
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Fecha fin congreso
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28/08/2016 |
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Desde la página
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204 |
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Hasta la página
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204 |
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Título de las actas
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12th European Nitrogen Fixation Conference |