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Descripción
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| Copper is a common redox cofactor for many physiological processes in plants, including photosynthesis, mitochondrial respiration, and symbiotic nitrogen fixation (SNF). The latter is the conversion, fixation, of N2 into NH3, a process carried out by endosymbiotic bacteria (rhizobia) in legume root nodules. SNF requires relatively large amounts of copper as an essential cofactor of some of the key reactions being carried out by the endosymbiotic rhizobia (Preisig et al., 1996). This oligonutrient is delivered by the host legume through the vasculature to the nodules and released in the infection/differentiation zone of them (where rhizobia differentiate into nitrogen-fixing bacteroids). From there, a plasma membrane transporter has to introduce copper into the cell for further cuproprotein assembly (Rodríguez-Haas et al., 2013). COPT family of copper transporters, mediate high affinity copper transport into the cytosol of eukaryotic cells (Peñarrubia et al., (2015); Sancenón et al., (2003)). From the eight COPT family genes present in M. truncatula genome, MtCOPT1 is the only one induced specifically in nodule. MtCOPT1 is able to restore Saccharomyces cerevisae ?ctr1 (defective in copper uptake) capacity to transport copper. Inmunolocalization and GUS fusion studies localize MtCOPT1 mainly in the plasma membrane of cells in the infection/differenciation zone of the nodule. In addition, a Tnt-1-derived knockdown mutant line for MtCOPT1 shows decreased nitrogenase activity when compared with the wild-type line. This activity is, at least, partially rescued when a wild-type copy of MtCOPT1 gene is reintroduced. Taken together, these data suggest an important role of MtCOPT1 copper-mediated transport for SNF. | |
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Internacional
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No |
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Nombre congreso
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XIII REUNIÓN DE BIOLOGÍA MOLECULAR DE PLANTAS |
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Tipo de participación
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970 |
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Lugar del congreso
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Revisores
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Si |
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ISBN o ISSN
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0000000000 |
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DOI
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Fecha inicio congreso
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22/06/2016 |
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Fecha fin congreso
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24/06/2016 |
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Desde la página
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161 |
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Hasta la página
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161 |
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Título de las actas
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XIII REUNIÓN DE BIOLOGÍA MOLECULAR DE PLANTAS |