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Memorias de investigación
Ponencias en congresos:
MtNramp1, MtZIP6, and MtCOPT1 are Respectively Responsible for Iron, Zinc, and Copper Uptake by Medicago truncatula Nodule Cells
Año:2016
Áreas de investigación
  • Ciencias naturales y ciencias de la salud
Datos
Descripción
Legume nodules require relatively large amounts of transition metals (iron, copper, zinc?) as cofactors of some of the key enzymes involved in symbiotic nitrogen fixation (1). These metals are provided by the host plant through the vasculature and, in the case of indeterminate type nodules, released in the infection/differentiation zone of the nodule (2). Consequently, a number of metal transporters must be in place to introduce these elements into the nodule cells to be later delivered to the symbiosomes. We have identified three Medicago truncatula metal transporters encoding genes, MtNramp1 (3), MtZIP6, and MtCOPT1, that are expressed at high levels in the nodule zone where metals have to be incorporated from the apoplast. Yeast complementation assays show that they are respectively involved in iron, zinc, and copper transport towards the cytosol. Consistent with a role in metal uptake from the apoplast, they are located in the plasma membrane of nodule cells. Knock out or knock-down plants of each gene have reduced growth and reduced nitrogenase activity. These phenotypes were restored by reintroducing a wild type form of the mutated gene or by watering the plants with a nutritive solution fortified in the corresponding transported metal. These results are consistent with a model in which MtNramp1 is responsible for Fe2+ loading of nodule cells, while MtZIP6 is for Zn2+ uptake, and MtCOPT1 for Cu+.
Internacional
Si
Nombre congreso
12th European Nitrogen Fixation Conference
Tipo de participación
960
Lugar del congreso
Revisores
Si
ISBN o ISSN
0000000000
DOI
Fecha inicio congreso
25/08/2016
Fecha fin congreso
28/08/2016
Desde la página
123
Hasta la página
123
Título de las actas
12th European Nitrogen Fixation Conference
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Manuel Tejada Jiménez (UPM)
  • Autor: Angela Enriqueta Saez Somolinos (UPM)
  • Autor: Marta Senovilla Ramos (UPM)
  • Autor: M del Rosario Castro Rodriguez (UPM)
  • Autor: Isidro Abreu (UPM)
  • Autor: Viviana Pamela Escudero Welsch (UPM)
  • Autor: Igor Kryvoruchko (Noble Foundation)
  • Autor: M. Mercedes Lucas (CSIC)
  • Autor: Michael Udvardi (Noble Foundation)
  • Autor: Juan Imperial Ródenas (UPM)
  • Autor: Manuel Gonzalez Guerrero (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
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