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Ponencias en congresos:
MtMOT1.3 Mediates Molybdenum Transport to Rhizobia-infected Medicago truncatula Nodule Cell
Año:2016
Áreas de investigación
  • Ciencias naturales y ciencias de la salud
Datos
Descripción
Symbiotic nitrogen fixation (SNF) requires molybdenum for nitrogenase to work, as an integral element of the FeMo cofactor. This metal has to be delivered from the host plant to the bacteroids in order to synthesize the cofactor. However very little is known about the transporters mediating molybdenum supply to nodules of legumes in connection to SNF. MOT1 family has been found to transport molybdate in plants (1), what maked them good candidates to be involved in molybdenum delivery to nitrogen-fixing bacteroids. We have identified a Medicago truncatula MOT1 member (MtMOT1.3) as responsible for molybdenum transport into rhizobia-infected cells. Heterologous expression in Saccharomyces cerevisiae shows that MtMOT1.3 is able to transport molybdate toward the cytosol. Its coding gene is mainly expressed in M. truncatula nodules, being 10-fold higher than the expression showed in roots or shoots. Immunolocalization studies show that MtMOT1.3 is located in the plasma membrane of infected and non-infected nodule cells. A loss of function mot1.3 mutant exhibited reduced growth under symbiotic conditions, associated with a decreased nitrogenase activity compared to wild-type plants. This phenotype was rescued by the addition of molybdate to the nutritive solution or by genetic complementation with a wild-type MtMOT1.3 copy. These results point to a role of MtMOT1.3 in molybdenum supply nodule cells, being a first step to understand Mo homeostasis in the legume-rhizobium symbiosis.
Internacional
Si
Nombre congreso
12th European Nitrogen Fixation Conference
Tipo de participación
970
Lugar del congreso
Budapest
Revisores
Si
ISBN o ISSN
0000000000
DOI
Fecha inicio congreso
25/08/2016
Fecha fin congreso
28/08/2016
Desde la página
286
Hasta la página
286
Título de las actas
12th European Nitrogen Fixation Conference
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Manuel Tejada Jiménez (UPM)
  • Autor: Juan Imperial Ródenas (UPM)
  • Autor: Manuel Gonzalez Guerrero (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
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