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Descripción
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| Symbiotic nitrogen fixation (SNF) requires molybdenum for nitrogenase to work, as an integral element of the FeMo cofactor. This metal has to be delivered from the host plant to the bacteroids in order to synthesize the cofactor. However very little is known about the transporters mediating molybdenum supply to nodules of legumes in connection to SNF. MOT1 family has been found to transport molybdate in plants (1), what maked them good candidates to be involved in molybdenum delivery to nitrogen-fixing bacteroids. We have identified a Medicago truncatula MOT1 member (MtMOT1.3) as responsible for molybdenum transport into rhizobia-infected cells. Heterologous expression in Saccharomyces cerevisiae shows that MtMOT1.3 is able to transport molybdate toward the cytosol. Its coding gene is mainly expressed in M. truncatula nodules, being 10-fold higher than the expression showed in roots or shoots. Immunolocalization studies show that MtMOT1.3 is located in the plasma membrane of infected and non-infected nodule cells. A loss of function mot1.3 mutant exhibited reduced growth under symbiotic conditions, associated with a decreased nitrogenase activity compared to wild-type plants. This phenotype was rescued by the addition of molybdate to the nutritive solution or by genetic complementation with a wild-type MtMOT1.3 copy. These results point to a role of MtMOT1.3 in molybdenum supply nodule cells, being a first step to understand Mo homeostasis in the legume-rhizobium symbiosis. | |
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Internacional
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Si |
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Nombre congreso
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12th European Nitrogen Fixation Conference |
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Tipo de participación
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970 |
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Lugar del congreso
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Budapest |
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Revisores
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Si |
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ISBN o ISSN
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0000000000 |
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DOI
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Fecha inicio congreso
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25/08/2016 |
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Fecha fin congreso
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28/08/2016 |
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Desde la página
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286 |
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Hasta la página
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286 |
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Título de las actas
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12th European Nitrogen Fixation Conference |