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Memorias de investigación
Artículos en revistas:
TWISTED DWARF1 Mediates the Action of Auxin Transport Inhibitors on Actin Cytoskeleton Dynamics
Año:2016
Áreas de investigación
  • Biología molecular, celular y genética
Datos
Descripción
Plant growth and architecture is regulated by the polar distribution of the hormone auxin. Polarity and flexibility of this process is provided by constant cycling of auxin transporter vesicles along actin filaments, coordinated by a positive auxin-actin feedback loop. Both polar auxin transport and vesicle cycling are inhibited by synthetic auxin transport inhibitors, such as 1-N-naphthylphthalamic acid (NPA), counteracting the effect of auxin; however, underlying targets and mechanisms are unclear. Using NMR, we map the NPA binding surface on the Arabidopsis thaliana ABCB chaperone TWISTED DWARF1 (TWD1). We identify ACTIN7 as a relevant, although likely indirect, TWD1 interactor, and show TWD1-dependent regulation of actin filament organization and dynamics and that TWD1 is required for NPA-mediated actin cytoskeleton remodeling. The TWD1-ACTIN7 axis controls plasma membrane presence of efflux transporters, and as a consequence act7 and twd1 share developmental and physiological phenotypes indicative of defects in auxin transport. These can be phenocopied by NPA treatment or by chemical actin (de)stabilization. We provide evidence that TWD1 determines downstream locations of auxin efflux transporters by adjusting actin filament debundling and dynamizing processes and mediating NPA action on the latter. This function appears to be evolutionary conserved since TWD1 expression in budding yeast alters actin polarization and cell polarity and provides NPA sensitivity.
Internacional
Si
JCR del ISI
Si
Título de la revista
Plant Cell
ISSN
1040-4651
Factor de impacto JCR
9,338
Información de impacto
Datos JCR del año 2014
Volumen
28
DOI
10.?1105/?tpc.?15.?00726
Número de revista
4
Desde la página
930
Hasta la página
948
Mes
ABRIL
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Jinsheng Zhu (University of Fribourg)
  • Autor: Aurelien Bailly (University of Fribourg)
  • Autor: Marta Zwiewka (Central European Institute of Technology)
  • Autor: Valpuri Sovero (University of Zurich)
  • Autor: Martin Di Donato (University of Fribourg)
  • Autor: Pei Ge (University of Fribourg)
  • Autor: Jacqueline Oehri (University of Fribourg)
  • Autor: Bibek Aryal (University of Fribourg)
  • Autor: Pengchao Hao (University of Fribourg)
  • Autor: Miriam Linnert (Max Planck Research Unit for Enzymology of Protein Folding)
  • Autor: Noelia Inés Burgardt (Max Planck Research Unit for Enzymology of Protein Folding)
  • Autor: Christian Lücke (Max Planck Research Unit for Enzymology of Protein Folding)
  • Autor: Matthias Weiwad (Max Planck Research Unit for Enzymology of Protein Folding)
  • Autor: Max Michel (Institute of Complex Systems)
  • Autor: Oliver H. Weiergräber (Institute of Complex Systems)
  • Autor: Stephan Pollmann (UPM)
  • Autor: Elisa Azzarello (LINV-DIPSAA)
  • Autor: Stefano Mancuso (LINV-DIPSAA)
  • Autor: Noel Ferro (University of Bonn)
  • Autor: Yoichiro Fukao (Nara Institute of Science and Technology)
  • Autor: Céline Hoffmann (Luxembourg Institute of Health)
  • Autor: Roland Wedlich-Söldner (University of Münster)
  • Autor: Jiri Friml (Institute of Science and Technology Austri)
  • Autor: Clément Thomas (Luxembourg Institute of Health)
  • Autor: Markus Geisler (University of Fribourg)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
S2i 2022 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)