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Abstract
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| Epigenetic mechanisms have been proposed to synchronize dormancy-growth transition in trees by generating reversible patterns of epigenetic marks (1, 2). Dormancy consists of growth cessation through the arrest of meristem activity and consequent transformation of the apical meristem into a dormant apical bud. A progressive demethylation of dormant apical bud has been suggested to play a role during the reactivation of growth, bud break (3, 4). However, so far no chromatin remodelers have been functionally related to bud break. In this work we report the identification of two poplar DEMETER-Like genes: PtaDML8 and PtaDML10. Our RT-PCR analyses showed that these genes are highly induced during bud break. Phenological assays showed that PtaDML8-10 knockdown plants (KD) have a delayed bud break. Transcriptome analyses revealed that these KD plants have altered biological processes such as cellular metabolic process, photosynthesis, ribosome biogenesis and response to light or temperature stimulus. We have also identified DMRs (differentially methylated region) in KDs vs. WT plants at bud break. This work shows, for the first time, that PtaDML8 and PtaDML10 are involved in active DNA demethylation required for bud break and growth resume. | |
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International
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Si |
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Congress
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Workshop New Frontiers in Plant Biology |
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970 |
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Place
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Centro de Biotecnología y Genómica de Plantas, Pozuelo de Alarcón, Madrid, España |
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Reviewers
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Si |
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ISBN/ISSN
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0000-0000 |
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Start Date
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15/06/2016 |
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End Date
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17/06/2016 |
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From page
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10 |
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To page
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10 |
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DNA demethylases promote bud break in poplar |